含微乳头结构肺腺癌CT重建征象特征及上皮间质转化相关基因表达

doi:10.16098/j.issn.0529-1356.2019.01.011

解剖学报 ›› 2019, Vol. 50 ›› Issue (1): 63-71.doi: 10.16098/j.issn.0529-1356.2019.01.011

含微乳头结构肺腺癌CT重建征象特征及上皮间质转化相关基因表达

周吉航¹ 续力云¹陆畅畅¹ 黄燕燕¹王昕晨¹乐涵波^2*

1.舟山医院细胞分子生物学实验室; 2.舟山医院胸心外科，浙江舟山 316021

收稿日期:2018-03-20 修回日期:2018-09-10 出版日期:2019-02-06 发布日期:2019-04-18
通讯作者: 乐涵波 E-mail:jeonchou@163.com
基金资助:
浙江省科技厅分析测试科技计划项目;浙江省医药卫生科技计划一般项目;舟山市公益类科技项目

Features of CT reconstruction images and expressions of epithelia-mesenchymal transition related genes in lung adenocarcinoma with micropapillary pattern

ZHOU Ji-hang¹ XU Li-yun¹ LU Chang-chang¹ HUANG Yan-yan¹ WANG Xin-chen¹ LE Han-bo ^2*

1.Laboratory of Cell and Molecular Biology; 2.Department of Cardiothoracic Surgery, Zhoushan Hospital, Zhejiang Zhoushan 316021, China

Received:2018-03-20 Revised:2018-09-10 Online:2019-02-06 Published:2019-04-18
Contact: LE Han-bo E-mail:jeonchou@163.com

摘要/Abstract

摘要：

目的探讨含微乳头结构（MPP）肺腺癌具有的CT重建征象特征，上皮间质转化（EMT）相关分子表达情况以及它们之间的关系。方法选取含MPP侵袭性肺腺癌（IAC）37例、不含MPP 80例及其对应的癌旁组织，比较临床病理特征、CT重建征象；应用Real-time PCR及Western blotting检测EMT有关标识分子表达变化情况。结果含MPP组淋巴结转移比例较高、毛刺、胸膜凹陷征、实性及分叶比例较高（均P<0.001）。含MPP组E-钙黏蛋白（cadherin）及β-连环蛋白（β-catenin）表达均下调（P<0.05），N-cadherins、波形蛋白（vimentin）、Snail及转化生长因子（TGF）-β表达均上调（P<0.05）。有毛刺组Ecadherin及β-catenin表达下调者所占比例较高，而vimentin表达上调者所占比例较高（均P<0.05）；实性结节组E-cadherin表达下调者所占比例较高，而N-cadherin及vimentin表达上调者所占比例较高（均P<0.05）。结论含MPP浸润性肺腺癌具有特征性CT重建征象，发生了EMT改变。其CT重建征象与EMT相关分子表达存在联系。

关键词: 微乳头结构, 上皮间质转化, 肺腺癌, 实时定量聚合酶链反应, 免疫印迹法, 人

Abstract:

Objective To investigate the features of CT reconstruction imags, expressions of genes associated with epithelial-mesenchymal transition(EMT) in lung adenocarcinoma with micropapillary pattern (MPP) and the relationships between them. Methods Thirty-seven cases of invasive lung invasive adenocarcinoma (IAC) with MPP, 80 cases without MPP and their corresponding para-cancerous tissues were selected. The clinicopathological features and features of CT reconstruction images were compared. Real-time PCR and Western blotting were used to detect the expression of EMT markers. Results In MPP group, the proportions of lymph node metastasis, spicule, pleural indentation, solid type and lobulation were higher（P<0.001）.In MPP group, the expressions of E-cadherin, β-catenin were decreased（P<0.05）, and the expressions of N-cadherin, vimentin,Snail and transforming growth factor(TGF)-β were increased（P<0.05）. In spicule group, the percentage of decreased expression of E-cadherin and β-catenin was higer, while the percentage of increased expression of vimentin was higher（P<0.05）. In the group of solid type, the percentage of decreased expression of E-cadherin was higher, while the percentages of increased expression of N-cadherin and vimentin were higher（P<0.05）. Conclusion Lung invasive adenocarcinoma has characteristics of CT reconstruction images and EMT changes. CT reconstruction images are associated with the expression of the related molecules associated of EMT.

Key words: Micropapillary pattern, Epithelial-mesenchymal transition, Lung adenocarcinoma, Real-time PCR, Western blotting, Human

周吉航续力云陆畅畅黄燕燕王昕晨乐涵波. 含微乳头结构肺腺癌CT重建征象特征及上皮间质转化相关基因表达[J]. 解剖学报, 2019, 50(1): 63-71.

ZHOU Ji-hang XU Li-yun LU Chang-chang HUANG Yan-yan WANG Xin-chen LE Han-bo. Features of CT reconstruction images and expressions of epithelia-mesenchymal transition related genes in lung adenocarcinoma with micropapillary pattern[J]. Acta Anatomica Sinica, 2019, 50(1): 63-71.

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含微乳头结构肺腺癌CT重建征象特征及上皮间质转化相关基因表达

Features of CT reconstruction images and expressions of epithelia-mesenchymal transition related genes in lung adenocarcinoma with micropapillary pattern

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