Abstract:

Objective To investigate the effect of dihydromyricetin（DMY）on proliferation and migration in choriocarcinoma JEG-3 and JAR cells. Methods JEG-3 and JAR cells were treated with different concentrations (0 mg/L, 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L) of DMY for 24 hours and 48 hours, and the proliferation was analyzed by methylthio tetrazole (MTT) assay. The effect of DMY on migration was detected by wound healing (after 24 hours) and Transwell assay (after treated JEG-3 for 36 hours and JAR for 24 hours). The expression of matrix metalloproteinase 2 (MMP-2) at mRNA and protein levels with different concentrations of DMY(0 mg/L, 40 mg/L, 60 mg/L, 80 mg/L) were detected by Real-time PCR (after 12 hours,24 hours,36 hours,48 hours)and Western blotting (after treated 36 hours) respectively. Results The proliferation of JEG-3 and JAR cells was inhibited significantly (P＜0.05), after DMY treatment for 24 hours and 48 hours.DMY inhibited the migration of JEG-3 and JAR cells significantly (P＜0.05) with a dose-dependent manner. After JEG-3 and JAR Cells treated with DMY for 36 hours and 48 hours, the expression of MMP-2 mRNA decreased significantly (P＜0.05),there were no significantly changes in DMY treatment with 12 hours and 24 hours. The expression level of MMP-2 protein was inhibited significantly after treatment with DMY for 36 hours (P＜0.05). Conclusion DMY might inhibit the proliferation in choriocarcinoma JEG-3 and JAR cells with a dose-dependent manner. The invasion and migration were inhibited by DMY through downregulation of MMP-2.

Key words: Choriocarcinoma, Dihydromyricetin, Proliferation, Migration, Transwell, Real-time PCR