Acta Anatomica Sinica ›› 2019, Vol. 50 ›› Issue (6): 713-717.doi: 10.16098/j.issn.0529-1356.2019.06.002

• Neurobiology • Previous Articles     Next Articles

Effect and mechanism of rosuvastatin preconditioning on inflammatory cytokines release in vascular smooth muscle cells of middle cerebral artery of rats after focal cerebral ischemia-reperfusion

LIU Ya-jun WANG Xuan* HU Hai GUI Yan   

  1. School of Medical Technology and Nursing Science,Wuhan Polytechnic University,Wuhan 430023, China
  • Received:2018-11-13 Revised:2018-12-17 Online:2019-12-06 Published:2019-12-06
  • Contact: WANG Xuan E-mail:wangxuan@126.com

Abstract:

Objective To explore the effect and probable mechanism of rosuvastatin preconditioning on inflammatory cytokines interleukin (IL)-1β,IL-6 and tumor necrosis factor α (TNF-α) release in vascular smooth muscle cells (VSMCs) of middle cerebral artery after ischemia-reperfusion. Methods Thirty-six healthy SD rats were randomly assigned into three groups:sham operation group,focal cerebral ischemia-reperfusion group and rosuvastatin preconditioning group. There were 12 rats in each group. At the 24th hour of reperfusion after middle cerebral artery occlusion (MCAO) for 2 hours, the mRNA and protein expression of IL-1β,IL-6 and TNF-α release in VSMCs of middle cerebral artery were detected by Real-time PCR and Western blotting,respectively.Also,the mRNA and protein expression of nuclear factor κB (NF-κB) were measured by Real-time PCR and Western blotting. Results At the 24th hour of reperfusion after MCAO for 2 hours,the mRNA and protein expression of IL-1β,IL-6 and TNF-α were markedly up-regulated in rats of model group;rosuvastatin preconditioning can significantly inhibited overexpression of IL-lβ,IL-6 and TNF-α at the mRNA and protein levels. And the decreasing of mRNA and protein expression of NF-κB was also found in this study. Conclusion Rosuvastatin preconditioning can decrease the release of inflammatory cytokines IL-1β,IL-6 and TNF-α in VSMCs of MCA. It can relieve the inflammatory injury after ischemia-reperfusion in brain. The effect of rovastatin on IL-1β,IL-6 and TNF-α may be related to the reduction of the expression of NF-κB in VSMCs.

Key words: Rosuvastatin, Vascular smooth muscle cell, Interleukin, Tumor necrosis factor α, Nuclear factor κB, Ischemia-reperfusion, Real-time PCR, Western blotting, Rat