AAS ›› 2017, Vol. 48 ›› Issue (5): 556-560.doi: 10.16098/j.issn.0529-1356.2017.05.010

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Expression of CXC chemokine receptor 4 and fork box protein 3 gene protein and mRNA in children mediastinal neuroblastoma cell strains SK-N-SH and LAN-5 and their effects induced by cyclophosphamide

XIE Yan-li WANG Tao*   

  1. Cardio-Thoracic Surgery,Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare)Hospital), Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430000, China]
     
  • Received:2017-03-22 Revised:2017-04-15 Online:2017-10-06 Published:2017-10-06
  • Contact: WANG Tao E-mail:xieyanli9988@163.com

Abstract:

Objective To observe the effect of cyclophosphamide on the CXC chemokin receptor 4(CXCR4) and fork box protein 3(Foxp3) expression of mediastinal neuroblastoma cell strains LAN-5 and SK-N-SH. Methods The Real-time PCR method was used to detect the relative expression of CXCR4 and Foxp3 mRNA, cell proliferation was detected by MTT, CXCR4 and Foxp3 gene expressions were analyzed by flow cytometry. Results CXCR4 and Foxp3 were high expressed in LAN-5 and SK-N-SH cells; IC50 of cyclophosphamide on LAN-5 and SK-N-SH cells were 6.5μmol/L and 3.9μmol/L, respectively; Cyclophosphamide decreased CXCR4 protein expression significantly in LAN-5 cell (P<0.01), also decreased the CXCR4 mRNA expression significantly in SK-N-SH cell (P<0.05), and cyclophosphamide downregulated Foxp3 protein (P<0.05) and Foxp3 mRNA (P<0.01) expression significantly in LAN-5 cells. Conclusion CXCR4 and Foxp3 may be a potential target for neuroblastoma chemotherapy.

Key words: Cyclophosphamide, Neuroblastoma, CXC chemokin receptor 4, Fork box protein 3, Real-time PCR, Child