Acta Anatomica Sinica ›› 2020, Vol. 51 ›› Issue (4): 543-547.doi: 10.16098/j.issn.0529-1356.2020.04.011

• Cancer Biology • Previous Articles     Next Articles

Dauricine inhibiting the cell proliferation and inducing the cell apoptosis of human pancreatic cancer cells line SW1900#br#

ZHANG Xiang1 FAN Jing2 ZHANG Yi-fen1 LI Hui1 LI Chang-yin3*   

  1. 1.Department of Pathology, Jiangsu Province Hospit of Chinse Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China;  2.Department of Clinical Education, AOMA Graduate School of Integrative Medicine, Texas 78745, USA; 3.Clinical Pharmacology Laboratory, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China
  • Received:2019-02-27 Revised:2019-03-29 Online:2020-08-06 Published:2020-08-06
  • Contact: LI Chang-yin E-mail:zx34892@163.com

Abstract:

Objective To discuss the proliferation inhibition and apoptosis induction of human pancreatic cancer cell line SW1900 by dauricine and its possible mechanism.   Methods The MTT colorimetric method  was used to detect the inhibitory effects of cell viability. The apoptosis rate was tested by the Annexin Ⅴ-FITC/PI fluorescent staining of flow cytometric method . The expressions of phosphatidylinositol 3-kinase (PI3K), protein kinase B(Akt) and B-cell lymphoma-2 (Bcl-2) were detected by Real-time PCR and Western blotting assay.  Results MTT assay showed that dauricine significantly inhibited the proliferation of SW1900 cells and the inhibitory effect was enhanced with the increasing of dauricine concentration, F=783.7, P<0.001. The apoptosis of 3 groups cells were (4.34±1.30)% (0 mg/L dauricine), (14.94±1.94)% (6 mg/L dauricine) and (22.68±3.61)% (12 mg/L dauricine). The mean difference was statistically significant among the three groups (F=58.52, P<0.001). Dauricine could significantly induce apoptosis human pancreatic cancer cells with dose-dependent manner. Real-time PCR showed that the gene expressions of PI3K, Akt and Bcl-2 were lower obviously (PI3K mRNA, F=101, P=0.01; Akt mRNA, F=1666, P<0.01; Bcl-2 mRNA, F=753, P<0.001) with dose-dependent manner. Western blotting assay also showed that the protein expression of PI3K, Akt and Bcl-2 was down-regulated with dose-dependent manner.  Conclusion Dauricine has proliferation inhibition and apoptosis inducement effect on human pancreatic cancer cells line SW1900. This function may be concerned with the regulation of PI3K/Akt signal pathway and lower Bcl-2 expression. 

Key words: Dauricine, Pancreatic cancer, SW1900 cell, Phosphatidylinositol 3-kinase, Protein kinase B, Bcl-2 gene, Western blotting, Human

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