Acta Anatomica Sinica

AAS ›› 2013, Vol. 44 ›› Issue (4 ): 576-579.doi: 10.3969/j.issn.0529-1356.2013.04.025

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Improvement of Masson staining in renal fibrosis of mice

WEI Sheng-nan ZENG Xiang-jun LI Hui-hua*   

  1. Department of Pathology and Pathophysiology, Capital Medical University, Beijing 100069, China
  • Received:2012-08-10 Revised:2013-01-05 Online:2013-08-06 Published:2013-09-04

Abstract:

Objective To get an improved Masson 3 staining method for staining renal fibrosis. Methods Bouin‘s fluid was used to re-fix the paraffin fixed sections. Ethanol∶acetic acid（2∶1) which was stored at -20℃ was used to treat the sections;
Brilliant green was used to substitute aniline blue. Duration of brilliant green staining was decreased. Results Renal interstitium of fibrosis and non-fibrosis were distinguished. Collagen fibers appeared green (counter staining by brilliant green) and cytoplasm
and muscle fibers were red. Conclusion In improved Masson 3 staining, tissues of fibrosis and non-fibrosis are distinguished by brilliant green, the staining results are stable and uniform. The method demonstrates a satisfied compared staining result.

Key words: Kidney, Fibrosis, Masson staining method, Mouse