Improvement of the method for in vitro culture of mouse embryos

doi:10.16098/j.issn.0529-1356.2015.01.023

Abstract

Abstract:

Objective To improve the embryo culture system for the quality of embryos and developmental potential. Methods In this study a rotating plastic capillary with an inside diameter of 0.21mm and an outside diameter of 0.28 mm were used to mimic oviduct properties and culture embryo in vitro. The capillary was inserted into a pipette tip, and then one to ten mouse 2-cell embryos in KSOM medium were drawn into a capillary by pipette. The capillary was immersed in a beaker containing a certain amount of autoclaved distilled water on a magnetic stirrer and then the device was placed in an incubator. After 48 hours culture the embryos were withdrew and their developmental potential were detected. Results Eighty-five and eight-two two-cells embryo were cultured in capillaries and microdrops, respectively. The blastocyst rate and average cell number are significantly higher in capillaries (85.4% and 57.0) than in microdrops (36.5%and 20.6). Capillary-cultured embryos formed bigger outgrowths than that of microdrop-cultured ones when they were seeded on matrigel-treated dishes. Conclusion Capillary culture, a method for in vitro culture the mouse embryo, raises success rates following human assisted reproduction.

Key words: Embryo, Microenvironment, Mechanical vibration, Capillary culture, In vitroculture, Mouse

SUN Fang-yuan DUAN Xin-chong ZHANG Zhen-nan ZHANG Xiao DI Ke-qian LI Xiang-yun*. Improvement of the method for in vitro culture of mouse embryos[J]. AAS, 2015, 46(1): 127-132.

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