Influence of three kinds of biological factors on the reproduction and regeneration in planarians

doi:10.16098/j.issn.0529-1356.2017.06.017

Abstract

Abstract:

Objective To investigate the effects of the three reproductive-relative factors on gonads and regeneration in planarians. Methods In this study, the planarians Dugesia japonica were cultured with three reproductive-relative factors: the hormones ［estradiol (E2), methyltestosteronum(MT)］, biogenic amine ［5-hydroxy-tryptamine (5-HT),dopamine (DA)］ and D-amino acids (D-tryptophan, D-arginine, D-phenylalanine). The cultured concentration was calculated by SPSS17.0 after the acute toxicity experiment, and the experimental phenomena were recorded. Results E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, which proportions were 20%, 40%, 20%, and 20% respectively, but DA made the gonads atrophy in 40% or 50% planarians. Besides, 5-HT increased speed of regeneration, while DA inhibited it during planarians regeneration. Conclusion E2, 5-HT, D-tryptophan and D-phenylalanine improved the gonads development, and 5-HT increased speed of regeneration; DA inhibited the gonads and regeneration.

Key words: Sexualization, Regeneration, Hormone, Biogenic amine, D-amino acid, Acute toxicity experiment, Dugesia japonica

DONG Zi-mei DONG Yan-ping CHEN Guang-wen LIU De-zeng. Influence of three kinds of biological factors on the reproduction and regeneration in planarians[J]. Acta Anatomica Sinica, 2017, 48(6): 732-739.

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