Effects of lilium soup on lung histological structure in mice injured by ephedrine

doi:10.16098/j.issn.0529-1356.2017.06.019

Abstract

Abstract:

Objective To explore the effects of lilium soup on ephedrine-injured mice lung. Methods Totally 72 Kunming mice ten days after birth were used and randomly divided into 3 groups：nature control group，experimental control group and lilium group．Experimental control group and lilium group were intraperitoneally injected escalation doses (2.0 g/L，4.0 g/L，6.0 g/L) ephedrine 0.2 ml twice every day．And nature control group was injected the same amount of saline．One hour after injection of ephedrine, lilium group was intragastric administration with 0.2 ml lilium soup．Experimental control group and nature control group were intragastric administed with the same amount of saline．The change of superoxide dismutase (SOD)，hydrogen peroxidase (CAT) and glutathione peroxidase (GSH-Px) activity of lung and the activity of plasma myeloperoxidase (MPO) were determined by colorimetry．HE staining was used to observe the change of histological structure of lung．Immunohistochemistry was used to detect the change of Bax and nuclear factor-kappa B (NF-κB) expression in lung． Results The activities of SOD，CAT and GSH-Px of experimental control group were significantly lower than that of the nature control group (P<0.01 or P<0.05)．The activity of plasma MPO of experimental control group were significantly increaser than that of the nature control group (P<0.01 or P<0.05)．Lung histopathologic damage of experimental control group was obvious．The alveolar structure were disorder and epithelial cells were fall off．The alveolar diameter and thickness of the alveolar septum increased significantly than the nature control group (P<0.01)．The stained color and average optical density of Bax and NF-κB increased significantly in experimental control group than that of the nature control group (P<0.01 or P<0.05)．Compared with experimental control group，the activities of SOD，CAT and GSH-Px of lilium group increased (P<0.01 or P<0.05). The activity of plasma MPO of lilium group reduced，the alveolar diameter and thickness of the alveolar septum were reduced in lilium group (P<0.01 or P<0.05) wih lighter histopathologic damage of lung，the stained color and the average absorbance of lilium group were reduced(P<0.01 or P<0.05). Conclusion Lilium soup could have an protective effect on the damage of mice lung induced by ephedrine by increase in activities of antioxidant enzyme and improving the body immunity，regulating the metabolism，restraining Bax and NF-κB expression of lung tissue and cell apoptosis．

LI Chong-yang PENG Jing YU Shi-yuan. Effects of lilium soup on lung histological structure in mice injured by ephedrine[J]. Acta Anatomica Sinica, 2017, 48(6): 746-752.

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