Effects of curcumin on adipogenic differentiation and expression of Krüppel like factor 2 in porcine adipose mesenchymal stem cells

doi:10.16098/j.issn.0529-1356.2017.05.007

Abstract

Abstract:

Objective To investigate the effects of curcumin on adipogenic differentiation and to reveal the mechanism of curcumin regulating porcine adipose mesenchymal stem cells(AMSCs) adipogenic differentiation. Methods The proliferation of porcine AMSCs was detected by MTT, the adipogenic differentiation was examined by oil red O staining extraction assay, and Krüppel like factor 2(KLF2) and peroxisome proliferator activatied receptor γ(PPARγ)2 mRNA expression patterns were detected by Real-time PCR after the porcine AMSCs were treated with various concentrations of curcumin. Results When various concentrations of curcumin were applied to treat the porcine AMSCs, 1μmol/L, 5μmol/L or 10μmol/L curcumin did not caused the significant effect on the cell proliferation, but higher than 15μmol/L curcumin had the significantly (P<0.05) inhibitory effect. The adipogenic differentiation of porcine AMSCs was detected, 1 to 30μmol/L curcumin significantly inhibited this progress, and 25μmol/L curcumin brought in the highest inhibiting rate (68.19%). When 25μmol/L curcumin was used to detect the mRNA expression patterns of KLF2 and PPARγ2 during porcine AMSCs adipogenic differentiation, the up-regulated rates of KLF2 were 48.37%, 20.56% and 9.64% and down-regulated rates of PPARγ2 were 16.01%, 35.93% and 27.27% on the day 2, day 8 and day 16 of adipogenic induction, respectively. Conclusion Curcumin inhibits the proliferation and adipogenic differentiation of porcine AMSCs, and this inhibitory effect might be associated with the upregulation of KLF2 mRNA expression and the downregulated transcription of PPARγ2.

Key words: Curcumin, Krüppel like factor 2, Adipose mesenchymal stem cell, Adipogenic differentiation, Real-time PCR, Pig

LIU Jing-xia LI Fang-zheng HUAN Yan-jun JIANG Zhong-ling SONG Xue-xiong. Effects of curcumin on adipogenic differentiation and expression of Krüppel like factor 2 in porcine adipose mesenchymal stem cells[J]. Acta Anatomica Sinica, 2017, 48(6): 675-681.

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