›› 2009, Vol. 40 ›› Issue (1): 7-12.doi: 10.3969/j.issn.0529-1356.2009.01.002

• 肿瘤基础研究专题报道 • Previous Articles     Next Articles

Preparation of magnetic gemcitabine stealth nanoliposomes and its antitumor effects EM>in vitro/EM>

  

  1. 1.Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology; 2.Pharmaceutical Preparation Section, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology; 3. Department of Laparoscope Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  • Received:2007-12-19 Revised:2008-02-14 Online:2009-01-06
  • Contact: TONG Qiang

Abstract: P>Objective To explore a new approach to prepare magnetic gemcitabine stealth nanoliposomes(MGSL), and evaluate its quality and its antitumor effects EM>in vitro/EM>.  Methods MGSL was prepared by reverse-phase evaporation method. The coated rate, shape, diameter, distribution of MGSL were examined by high performance liquid chromatography(HPLC), microscopy and laser light scattering. Then the stability of MGSLs was also evaluated. The antitumor effect on breast cancer cell line MCF-7 EM>in vitro/EM> was investigated. Electron microscopy was employed to observe morphological features and ultrastructure changes of apoptosis of MCF-7 cells. The effect of MGSL on proliferation inhibitory rate of MCF-7 cells was measured with MTT method. The FCM analysis was carried out to examine the cell cycle distribution and cell apoptotic rate. Results The electron microscope and atomic force microscope photographs of MGSL showed that the shape of liposome was round or elliptical. It was showed that 97.62 percent of the liposome optimized was 206.6nm with above the coated rate 81.7%. Moreover, it possessed a good magnetic response and a high stability. MCF-7 cell line was sensitive to MGSL and the cytotoxity was correlated with the loaded drug dose. After treated for 2h, the effect of MGSL on proliferation inhibitory rate of MCF-7 cells was lower than that of gemcitabine group(EM>P/EM><0.05), while treated for 48h, the effect on proliferation inhibitory rate was similar(EM>P/EM>>0.05). Moreover, the effect on proliferation was also similar between MGSL group and MGL group(EM>P/EM>>0.05).The effect of apoptosis of MGSL on MCF-7 was obvious and the rate of apoptosis was 51

Key words: Gemcitabine, Stealth Nanoliposomes, Drug carrier, EM>In vitro/EM>,  Antitumor effect, HPLC, MTT, Flow cytometry

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