Objective To explore the gender and regional differences of the nasal discrete traits in Han ethnic groups.   Methods From 2015 to 2019, eight nasal discrete traits were observed in three Han ethnic groups from Henan, Jiangsu and Guangxi, with sample size of 1022, 963, and 1017, respectively. The statistical tests were performed on gender and regional level, and the multiple correspondence analysis (MCA) was used to study the correlation between these morphological traits and regions.  Results Most nasal discrete traits showed significant statistical differences within gender, and exhibit differences in regional populations. In dimension 1 (Dim 1) of MCA figures, samples in Guangxi separate from the other two regions’, while there was considerable overlap between samples from Henan and Jiangsu. Among eight nasal discrete traits, the shape of nasal tip, alae nasi height and alae nasi projection contribute most to Dim1.  Conclusion Nasal tip and nasal wings exhibited significant regional differences in Han Chinese.