Objective  To investigate the role of cell division cycle associated 7 (CDCA7) in breast cancer and the potential mechanisms among the progressioned.   Methods  By data analysis in data base to retrieve the differential expression gene and find out highly expressed genes in basal-like breast cancer cells. Among these genes we selected the attractive CDCA7 as the target of investigation. Through specimen immunohistochemistry analysis of clinical patients to confirm the significance of the research. Then we constructed CDCA7 stable expression cell lines to preform a series of experiments including colony formation assays, tumorsphere formation assays, flow cytometry, Western blotting and Real-time PCR to clear the role of CDCA7 in breast cancer.   Results  Data base analysis and histochemistry showed CDCA7 was highly expressed in basal-like breast cancer cells and high expression of CDCA7 predicts poor prognosis. Overexpressing CDCA7 in luminal cell lines, the cell lines showed stronger proliferative capacity, more mitotic cell and less apoptotic cell. Meanwhile, the markers of stem cell, such as CD133 and acetaldehyde dehydrogenase(ALDH) showed being upregulated. The Real-time PCR and Western blotting analysis showed that CDCA7 increased the expression of β-catenin, through which CDCA7 effected the Wnt pathway so as to influence cell proliferation and stemness.   Conclusion  CDCA7 as a oncogenic gene induces luminal cell to transform to basal-like subtype by depending on β-catenin and Wnt pathway.