Objective To investigate the effect of mircoRNA-128-3p (miR-128-3p) on epithelial-mesenchymal transition (EMT) of ovarian cancer cells and its regulatory mechanism on zinc finger E-box binding homobox 1(ZEB1).   Methods Real-time PCR technology was used to detect the expression of miR-128-3p in epithelial ovarian cancer tissue (EOC) and adjacent normal tissue(30 cases each), and to observe whether there was a difference. Two human ovarian cancer cell lines, SKOV3 and A2780, were selected and transfected respectively. MiR-128-3p mimic (miR-128-3p mimic group) and negative control mimic (NC mimic group) were used to detect the expression of miR-128-3p in 4 groups by Real-time PCR to verify the interference effect. Transwell assay was used. The migration and invasion abilities of the four groups of cells were observed. The regulatory relationship between miR-128-3p and ZEB1 was verified by dual luciferase assay, and the expression level of ZEB1 protein after overexpression of miR-128-3p was detected by Western blotting; pcDNA-ZEB1 was transfected into SKOV3 and A2780 cell lines to make it overexpression of ZEB1 was divided into NC mimic group, miR-128-3p mimic group, and miR-128-3p mimic+pcDNA-ZEB1 group. Western blot  ting was used to detect the EMT-related protein E-cadherin in 6 groups of cells and the expression level of vimentin.   Results Real-time PCR result showed that the expression of miR-128-3p in EOC tissues decreased compared with that in adjacent tissues(P<0.01); The relative expression of miR-128-3p in the miR-128-3p mimic group was higher than that in the NC mimic group, while the numbers of migrating cells and invasive cells were lower than those in the NC mimic group (P<0.01). The result  of dual luciferase experiments showed that miR-128-3p had a negative regulatory effect on ZEB1. In SKOV3 and A2780 cells, the relative expression of ZEB1 protein in the miR-128-3p mimic group was lower than that in the NC mimic group, while the relative protein expression of Ecadhein was higher than that in the NC mimic group (P<0.01). The relative protein expression of E-cadhein in the miR-128-3p mimic+pcDNA-ZEB1 group was lower than that in the miR-128-3p mimic group (P<0.01). In SKOV3 and A2780 cells, the relative protein expression of vimentin in the miR-128-3p mimic group was lower than that in the NC mimic group, and the relative p  rotein expression of vimentin in the miR-128-3p mimic+pcDNA-ZEB1 group was higher than that in the miR-128-3p mimic group (P<0.01).   Conclusion The expression of miR-128-3p decreases in epithelial ovarian cancer tissues, which may be due to the regulation of ZEB1 to affect the expression of EMT-related proteins and participate in the EMT process of ovarian cancer cells.