Molecular analysis of interleukin 10 promoter region-592A/C polymorphism in hypertensive disorders of pregnancy in Qinghai Han nationality

doi:10.16098/j.issn.0529-1356.2023.01.009

Abstract

Abstract:

Objective To investigate the association between the interleukin 10 (IL-10) gene promoter region-592A/C (rs1800872) polymorphism and hypertensive disorders of pregnancy (HDP) in Han women of Qinghai province and to determine the expression of this gene in two groups (HDP group and healthy control group) preliminarily. Methods A total of 140 HDP patients (HDP group) and 140 normal pregnant women (control group) in Qinghai Province were selected. Using blood DNA as template, the IL-10-592A/C polymorphism typing of HDP group and control group was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and verified by sequencing. The expression of IL-10mRNA in the placental tissues of the two groups was detected by Real-time PCR. Plasma IL-10 levels of the two groups were detected by ELISA. Results The frequencies of AA, AC and CC genotypes of IL-10 gene in HDP group and control group were 24.29%, 44.29%, 31.42% and 13.57%, 41.43%, 45.00% respectively, the difference in genotype distribution between the two groups was statistically significant (P<0.05);AA genotype frequency in HDP group(24.29%)was higher than that of control group(13.57%)( P<0.05), CC genotype frequency in HDP group (31.42%) was lower than that in control group (45.00%) (P<0.05), while there was no significant difference in genotype frequency of AC between the two groups (P<0.05); The distribution of A and C allele frequencies of IL-10-592A/C polymorphism was different between the two groups, and the A allele frequency of HDP group was higher than that of control group (χ²=8.079, P<0.05); The expression of IL-10mRNA in the placental tissue of the HDP group was lower than that of the control group (P<0.01); The level of plasma IL-10 in HDP group (1.53 ± 0.68) ng/L was lower than that in control group (1.79 ± 0.72)ng/L (P<0.01). Conclusion IL-10-592A/C polymorphism is associated with HDP in Qinghai Han nationality. The A allele in IL-10-592A/C polymorphism may be involved in the genetic susceptibility to HDP by regulating the expression of IL-10.

Key words:

Hypertensive disorders of pregnancy, Interleukin 10, Genetic polymorphism, Cytokine, Real-time PCR, Polymerase chain reaction-restriction fragment length polymorphism, Pregnant woman

CLC Number:

DUAN Qian LI Jian-hua LI Wen-jia WANG Ru WU Zhen YUAN Ming.

Molecular analysis of interleukin 10 promoter region-592A/C polymorphism in hypertensive disorders of pregnancy in Qinghai Han nationality

[J]. Acta Anatomica Sinica, 2023, 54(1): 63-69.

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