Change of angiotensin-converting enzyme 2 expression in the macrophage foam cell induced by oxidized low density lipoprptein

Abstract

Abstract: Objective To investigate the change of ACE2 expression in the macrophage foam cell induced by ox-LDL. Methods Different concentrations of ox-LDL were used to induce macrophage cell RAW264.7 into foam cells, the intracellular lipid droplets amounts were observed by oil red O staining, the content of MDA was measured to identify oxidative damage, Real-Time PCR was used to detect ACE2 and MAS mRNA expression, the activity of SOD and the content of GSH were measured by chemocolorimetry. Results The macrophages became larger and rounder after treated by ox-LDL (20、40、60、80μg·ml-1) for 24h by oil red O staining, the amount of lipid droplets in the cytoplasm increased in a dose-dependent manner. The MDA content was increased significantly compare with the control group using 60μg·ml-1 ox-LDL treatment (P<0.01), Real-time PCR results showed that ACE2 and MAS mRNA expression in model group were decreased (P<0.05), and the SOD activity and the GSH content were also decreased (P<0.01 or P<0.05) significantly in model group. Conclusion Macrophage foam cell formation is closely related with the down-regulation of ACE2 and MAS expression, and that means ACE2 has anti-oxidative effect which may be related to SOD and GSH.

Key words: ox-LDL, Macrophage, Foam cell, ACE2, Anti-oxidative effect

References

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