Expression of phosphorylated-signal transduction and activator of transcription 3 (Y705) in adenomyosis and their clinical significances

doi:10.16098/j.issn.0529-1356.2016.02.020

Abstract

Abstract:

Objective To investigate the role of phosphorylated-signal transduction and activator of transcription 3（p-STAT3） (Y705) and vascular endothelial growth factor (VEGF) in the development of adenomyosis by detecting the expression of p-STAT3 (Y705) and VEGF in normal endometrium and eutopic endometrium of adenomyosis patients. Methods The localization and expression of p-STAT3 (Y705) and VEGF in 14 cases of normal endometrium and 14 cases of eutopic endometrium of adenomyosis patients were detected by immunohistochemical SP method.Real-time PCR was performed to understand the expression of signal transduction and activator of transcription 3（STAT3）mRNA in normal endometrium and eutopic endometrium of adenomyosis patients. The protein levels of p-STAT3(Y705) , STAT3 and VEGF in normal endometrium and eutopic endometrium of adenomyosis patients were detected by Western blotting. Results In the normal endometrium and eutopic endometrium of adenomyosis, p-STAT3 (Y705) was mainly located in the nucleus of the glandular epithelium. p-STAT3 (Y705) had higher expression in adenomyosis groups than that in control (P＜0.05). There was no significant difference in the expression of p-STAT3 (Y705) in the proliferative phase and secretory phase of each group (P＞0.05). In the normal endometrium and eutopic endometrium of adenomyosis, there was no significant difference in the expression of STAT3 mRNA and protein levels (P＞0.05). VEGF immunostaining mainly localized in the the glandular epithelial cells. In ectopic endometrium of adenomyosis, the expression of VEGF was significantly higher than the controls（P＜0.05). There was no significant difference in the expression of VEGF in the proliferative phase and secretory phase of each group (P＞0.05). Conclusion p-STAT3 (Y705) may play a role via promoting angiogenesis in the development of the adenomyosis.

WANG Jing YANG Yang YANG Ning DENG Xiao-hui YANG Xing-sheng CHAO Lan*. Expression of phosphorylated-signal transduction and activator of transcription 3 (Y705) in adenomyosis and their clinical significances[J]. AAS, 2016, (2): 261-267.

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