Acta Anatomica Sinica

AAS ›› 2016, Vol. ›› Issue (2): 152-158.doi: 10.16098/j.issn.0529-1356.2016.02.002

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Relationship between nerve cells, glial cells and blood vessels during the development of mouse cerebral cortex

CUI Zhan-jun1 BIAN Ke2 ZHAO Kai-bing2 SHI Shu-qin1 DENG Jin-bo 1*   

  1. 1. Institute of Neurobiology, Medical College of He’nan University，He’nan Kaifeng 475001，China; 2. Department of Anatomy, Medical College of Kaifeng University，He’nan Kaifeng 475001，China
  • Received:2015-08-20 Revised:2015-10-14 Online:2016-04-06 Published:2016-04-06
  • Contact: DENG Jin-bo E-mail:jinbo_deng@henu.edu.cn

Abstract:

Objective To investigate the relationship between nerve cells, glial cells and angiogenesis in mouse cerebral cortex development. Methods Brain neural stem cells, neural precursor cells, glial cells and blood vessels of embryonic 15 days (E15) to postnatal 90 days (P90) mice were labeled by immunofluorescence, 5-bromodeoxyuridine(BrdU) and ink perfusion for morphological observation. Results Around the embryonic period of E15, the cerebral cortex began to have vascular networks, and later with the growth of age, the vascular branches gradually increased, the density of the blood vessels showed an increasing trend, and the density of adult (P60) blood vessels was stable. The direction of the small blood vessels in the cerebral cortex was consistent with that of radial glial cells. A large number of radial glial cells extended enlargement of the footplate participating in construction of the blood brain barrier. The proliferation of neural stem cells migrated along the direction of the vessel and the extension direction of radial glial cells. Conclusion During the development of cerebral cortex, neural stem cells proliferate and migrate along the direction of blood vessels and radial glial cells. Nerve cells and glial cells are involved in the structure of the vessel wall, and the interaction among them is to maintain the integrity and function of the blood brain barrier.