Expression of integrin β1 and focal adhesion kinase of the left ventricule in renal hypertensive rats

doi:10.16098/j.issn.0529-1356.2016.03.018

Abstract

Abstract:

Objective To explore the variation of integrin β1 and focal adhesion kinase（FAK）during left ventricular wall re-modeling in the rats with renal hypertension.
Methods The animal model was prepared by bilateral renal artery stenosis, and renal artery was freed in the control group. Forty rats were randomly divided into sham operation group, and two-week group, five-week group and seven-week group after operation. The model quality of hypertensive rat was assessed by a noninvasive blood pressure analysis tester and the index of left ventricular weight (LVW/BW). The morphological changes of the left ventricle was observed under a light microscope after HE staining. The immunohistochemistry and Western blotting methods were used to detect integrin β1 and FAK distribution in the ventricle muscle tissues. Results HE staining showed that the myocardial fibers were disordered and the space between the myocardial cells increased. The positive signals of both of integrin β1 and FAK immunohistochemistry were distributed in myocardium. The intensities of the immunohistochemical positive staining profiles for both integrin β1 and FAK increased with time and reached equilibrium for 5weeks treatment by image analysis. Average absorbance(AA) value of FAK expression at the 2nd week was declined significantly than the control group and increased with time in operation groups. Western blotting showed that the expression level of integrin β1 and KAK in every operation group were all higher than sham group (P<0.01). Five-week group was declined significantly compared with 2-week group (P<0.01) and no significance difference between 7-week and 5-week group（P＞0.05）.FAK had a significantly lower expression level in all operation groups than control group, 5-week group was declined significantly compared with 2-week group (P<0.01) and no significance difference between 7-week and 5-week group（P＞0.05）. Conclusion The abnormal expression of integrin β1 and FAK in the left ventricular may be an indication of myocardial hypertrophy.

TIAN Xiang-qin LIU Ying-chun CAI Xin-hua*. Expression of integrin β1 and focal adhesion kinase of the left ventricule in renal hypertensive rats[J]. AAS, 2016, (3): 391-395.

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