Acta Anatomica Sinica

AAS ›› 2014, Vol. ›› Issue (2): 185-189.doi: 10.3969/j.issn.0529-1356.2014.02.008

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Stereoscopic study on the expression of Bcl-2 and Bax in development of mice hippocampus

ZHANG Li ZHANG Jing-kun GUO Min*   

  1. Teaching and Research Section of Histology and Embryology, Liaoning Medical College, Liaoning Jinzhou 121001, China
  • Received:2013-03-22 Revised:2013-04-28 Online:2014-04-06 Published:2014-04-06
  • Contact: GUO Min E-mail:ln_zhangli@hotmail.com

Abstract:

Objective To observe the expression of Bcl-2 and Bax in the development of C57/BL6 mice hippocampus. Methods Mice hippocampus of Embryos (E) 18 day (d), E 20 d, postnatal (P) 1 d, P 3 d, P 7 d, P 14 d, P 28 d, P 2 month (M), P 3 M, P 6 M, P 15 M, P 18 M were used in this study. Immunohistochemistry and stereoscopy were used to detect the expression of Bcl-2 and Bax protein in the mice hippocampus at different ages. Results From E18 d to P21 d, Bcl-2 and Bax immunoreactive cells in both Ammon’cornu (CA) and dentate gyrus (DG) firstly and gradually increased and then decreased（P<0.01）. The volume density of both Bcl-2 and Bax immunoreactive cells came to the top on P7 d except the Bax expression in DG which was the highest on P14 d（P<0.01）.
Conclusion The expression of Bcl-2 and Bax is high at the early stage of hippocampus development and then low at its adult stage and senectitude, which may be involved with the forming of hippocampus.