Acta Anatomica Sinica

AAS ›› 2014, Vol. ›› Issue (2): 176-180.doi: 10.3969/j.issn.0529-1356.2014.02.006

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Expression and significance of Nestin and SOX2 in the SAMP8 mice subfornical organ

YE Jian-ya1 LI Cong-hui2 TANG Wei-dong1 MA Chang-sheng3 HAO Qing-mao1 QIN Yong 1*   

  1. 1. Hebei Medical University Xishan Campus, Shijiazhuang 050200,China; 2.Department of Neurosurgery, the First Hospital of Shijiazhuang, Shijiazhuang 050000,China; 3.Laboratory of Neurological Biology，Hebei Medical University, Shijiazhuang 050017,China
     
  • Received:2013-07-22 Revised:2013-09-03 Online:2014-04-06 Published:2014-04-06
  • Contact: QIN Yong E-mail:sjzwxqy@126.com

Abstract:

Objective To observe the expression of nestin and sex-determining genes in high-mobility group proteins in the senescence accelerated mouse prone 8 subfornical organ. Methods Adult SAMP8 mice (8 months old) were used. The expressions of Nestin, SOX2 in subfornical organ were observed by using immunohistochemical staining and immunofluorescence staining methods. Normal senescence mouse resistance 1 was displayed as control. Results Nestin immunohistochemical staining of the control group: positive cells were protuberantly distributed and expressed filiform texture with radiate. Nestin immunohistochemical staining of the experimental group: positive cells body stained stronger, filiform texture was thick, densely distributed in surrounded by vessels. The percentage of positive cells（49.17±7.60）% increased significantly than control group（16.33±4.41）% and had statistical significance (P<0.01). SOX2 immunohistochemical staining of control group: positive cells stained irregularity and dispersedly distributed. SOX2 immunohistochemical staining of the experimental group: most of positive cells stained deeper, densely distributed in surrounded by vessels. The percentage of positive cells（62.17±20.27）% increased significantly than control group（36.00±16.20）% and had statistical significance (P<0.05). Fluorescence double staining in the control group: SOX2 stained irregularity and Nestin expressed during it. A small amount of SOX2 and Nestin double-positive cells were in the subependymal region. In the experimental group: positive cells expressed intensity and showed more double-positive cells. Conclusion Expression of nestin and SOX2 enhances significantly in the senescence accelerated mouse prone 8 subfornical organ, which indicates AD may result in increased number of neural stem/progenitor cell proliferation or differentiation in subfornical organ at same stage，and then the neurogenesis of subfornical organ may be affected in Alzheimer disease.