关键词: α7-尼古丁乙酰胆碱受体, EphB/Ephrin-B1, 睫状神经节神经元, 膜片钳技术, 鸡胚

Abstract: Objective Recent researches showed that Eph/Ephrin tyrosine kinase family plays an important role in the development and functional maintanence of nervous system, but it is unclear if it affects sympathetic nervous system.The study is to detect the effect of EphB/Ephrin-B1 signal on the currents of nicotinic acetylcholine receptor (α7-nAChRs) on ciliary ganglion neurons. Methods Currents of α7-nAChRs on cultured and acute-associated ciliary ganglion neurons were recorded respectively. Cells were randomly divided into four groups: the control group, the EphrinB1Fc treated group (cells stimulated with different concentrations of Ephrin-B1Fc which is a recombination of IgG and Ephrin-B1), IgG treated group (cells stimulated with IgG at the same concentration of IgG needed for Ephrin-B1Fc recombination), Ephrin-B1 treated group (cells stimulated with Ephrin-B1 at the same concentration of Ephrin-B1 needed for Ephrin-B1Fc recombination). Results There was no difference between the control, IgG and Ephrin-B1 groups, but Ephrin-B1Fc could inhibit α7-nAChRs currents of both cultured and acute-associated CG neurons in a concentration-dependent manner. The inhibition happened quickly and was reversible. ICSUB>50/SUB> of ISUB>peak/SUB>/CSUB>m/SUB> of cultured and acuteassociated cells was 0.032mg/L and 1.4mg/L respectively. Conclusion The results showed that EphB/Ephrin-B1 signal can affect currents of CG α7-nAChRs. Is suggests that

Key words: α7-nAChRs, EphB/Ephrin-B1, Ciliary ganglion neurons, Patch clamp technique, Chicken embryo