解剖学报

›› 2010, Vol. 41 ›› Issue (03): 458-461.doi: 10.3969/j.issn.0529-1356.2010.03.026

• 论著 • 上一篇    下一篇

成年小鼠海马结构的三维重建

吴辉群1; 蒋葵1 ;胡钰琛1; 熊旭东1; 白龙翔1 ;耿兴云1; 吕广明2; 董建成1*   

  1. 1.南通大学医学院医学信息学教研室，南通大学数字医学研究所, 江苏　南通 226001;2.南通大学医学院人体解剖学教研室，江苏省神经再生重点实验室, 江苏　南通 226001
  • 收稿日期:2009-06-05 修回日期:2009-10-09 出版日期:2010-06-06
  • 通讯作者: 董建成

Three-dimensional reconstruction of hippocampal formation in normal adult mice

  1. 1. Department of Medical Informatics, Institute of Digital Medicine, Medical School of Nantong University, Jiangsu Nantong 226001,China; 2.Department of Human Anatomy, Jiangsu Key Laboratory of Nerve Regeneration, Medical School of Nantong University, Jiangsu Nantong 226001,China
  • Received:2009-06-05 Revised:2009-10-09 Online:2010-06-06
  • Contact: DONG Jian-cheng

关键词: 海马结构, 连续切片, 3D-DOCTOR, 三维重建, 小鼠

Abstract: Objective To make three-dimensional (3D) reconstruction of mouse brain and inner hippcampal formation (HF) with 3D software, and to observe and measure the reconstructed structures. Methods The Nissl stained mouse brain serial cryosections were obtained and pre-processed to construct the image dataset for 3D reconstruction. Using PhotoshopCS3 software, the different anatomic structures of HF in mouse coronary brain sections were fulfilled with different colors according to anatomy knowledge. The 3D-DOCTOR 4.0 software was used to make registration, segmentation and 3D reconstruction of mouse brain and HF, and to observe the reconstructed structures in personal computer (PC). Results The serial Nissl stained cryosections could be used to make 3D reconstruction of mouse HF, and the subiculum, CA1, CA2, CA3, CA4, dentate gyrus (DG), etc, could be observed. Conclusion The serial Nissl stained mouse cryosections could be used to make 3D reconstruction of mouse brain and HF.

Key words: Hippocampal formation, Serial section, 3D-DOCTOR, Three-dimensional reconstruction, Mouse

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