解剖学报

›› 2010, Vol. 41 ›› Issue (4): 538-544.doi: 10.3969/j.issn.0529-1356.2010.04.011

• 论著 • 上一篇    下一篇

免疫抑制药对体外骨髓前体细胞向巨噬细胞分化的影响

沈红1， 2; 李龙3 ;赵勇4*    

  1. 1.北京农学院动物科技学院, 北京 102206; 2. 兽医学（中医药）北京市重点实验室，北京 102206; 3. 北京维通利华实验动物技术有限公司，北京 100107;4.中国科学院动物研究所生物膜与膜生物工程国家重点实验室, 北京 100101
  • 收稿日期:2009-11-14 修回日期:2009-11-30 出版日期:2010-08-06
  • 通讯作者: 赵勇

Effects of immunosuppressive drugs on bone marrow progenitor cells differentiating into macrophages EM>in vitro/EM>

  1. 1.Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China；2. Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing 102206, China；3. Beijing Vital River Laboratory Animal Technology Co. Ltd., Beijing 100107, China；4. State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Science, Beijing 100101, China
  • Received:2009-11-14 Revised:2009-11-30 Online:2010-08-06
  • Contact: ZHAO Yong

关键词: 雷帕菌素, 环孢菌素A, 紫杉醇, 骨髓前体细胞, 巨噬细胞, 诱导, 分化, 小鼠

Abstract: Objective To investigate the effects of immunospressive drugs supplemented in culture media which provided the microenvironment for the generation of marophages bone marrow progenitor cells (BMPCs) differentiating into macrophages. BR> Methods BMPCs were prepared asepticly from C57BL/6 mice killed by cervical dislocation and EM>in vitro/EM> cultured in the media containing macrophage colony-stimulating factor (M-CSF) and rapamycin (rapa), cyclosporin A (CsA) and paclitaxel respectively. The morphology and phenotype of induced macrophages from BMPCs were analyzed by microscopy and flow cytometry (FCM). Results Induced cells from BMPCs presented specific macrophage morphology. There were high expressions of F4/80 and CD11 in surface molecules of induced macrophages and a dramatically decreased expression of CD11c. However, compared with the control group, there was a significant decrease in counts of differentiating macrophages. There was no significant effect on cell cycle of induced macrophages from BMPCs treated with cyclosporin A and a significant effect on apoptosis of those treated with paclitaxel. BR> Conclusion Three immunosuppressive drugs used in the experiment may affect the development and differentiation of macrophages from BMPCs. BR>

Key words: Rapamycin, Cyclosporin A, Paclitaxel, Bone marrow progenitor cell, Macrophage, Induce, Differentiation, Mouse

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