解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (1 ): 97-101.doi: 10.3969/j.issn.0529-1356.2013.01.019

• 组织学胚胎学发育生物学 • 上一篇    下一篇

GRIM-19在小鼠植入前胚胎中的表达及其作用

崔文娟,邓晓惠,晁岚*,沈彦军,杨芳,冯文娟,徐静,陈红蕾   

  1. 山东大学齐鲁医院不孕不育诊疗中心，济南 250012
  • 收稿日期:2012-03-06 修回日期:2012-05-10 出版日期:2013-02-06 发布日期:2013-02-06
  • 通讯作者: 晁岚 E-mail:chaolan@sdu.edu.cn
  • 基金资助:

    国家自然科学基金（30901603），山东省优秀中青年科学家科研奖励基金（BS2009SW031），山东省人口和计划生育委员会科学技术研究项目（2009年第8号）;省自然科学基金资助项目

Expression of GRIM-19 and its effects in preimplantation embryo of mice

CUI Wen-juan,DENG Xiao-hui,CHAO Lan*,SHEN Yan-jun,YANG Fang,FENG Wen-juan,XU Jing, CHEN Hong-lei    

  1. Infertility Center, Qilu Hospital, Shandong University, Ji’nan 250012, China
  • Received:2012-03-06 Revised:2012-05-10 Online:2013-02-06 Published:2013-02-06

摘要：

目的 通过研究干扰素/维甲酸联合应用诱导细胞凋亡相关基因 (GRIM-19)在小鼠植入前胚胎中的表达及其与胚胎质量之间的相关性，探讨GRIM-19在小鼠植入前胚胎发育中的作用。方法 采用实时定量PCR，检测小鼠植入前胚胎（2-细胞期、4-细胞期、8-细胞期、桑葚胚和囊胚）中GRIM-19 mRNA水平 (n =16)；将小鼠8细胞期胚胎按卵裂球大小、形态、透明带、胞质碎片分为优胚组和非优胚组，分别检测两组胚胎GRIM-19 mRNA水平，并分析其相关性 (n =13)；制作假孕鼠（23只），并随机分为A组（12只）和B组（11只），采用移植器将优质和非优质8-细胞期胚胎移入假孕鼠的子宫内，观察两组雌鼠妊娠率及产仔率。结果 GRIM-19在小鼠植入前各期胚胎中均有表达，其mRNA水平从2-细胞期逐渐增高，至8-细胞期达高峰，随后呈下降趋势。优质8-细胞胚胎的GRIM-19 mRNA水平明显高于非优胚组（P <0.05）。两组8-细胞胚胎移植后，A组雌鼠妊娠率及产仔率显著高于B组雌鼠（P<0.05）。 结论 小鼠植入前胚胎中GRIM-19的表达量随发育时程的改变而变化，且与胚胎质量密切相关。

关键词: 干扰素/维甲酸联合应用诱导细胞凋亡相关基因, 植入前胚胎, 线粒体, 实时定量PCR, 小鼠

Abstract:

Objective To study the expression of gene associated with retinoid-interferon-induced mortality-19(GRIM-19) and its correlation with the embryo quality, and to explore the effect of GRIM-19 in the development of preimplantation embryo of mice. Methods The expression of GRIM-19 in 2-cell, 4-cell, 8-cell, morula and blastocyst of mice preimplantation embryo was detected by using real-time PCR (n =16). Eight-cell embryos were divided into two groups: good quality embryos and bad quality embryos, according to their size, the pellucid zone and the debris of neoplasm. The different levels of embryos were detected (n=13). The expression of GRIM-19 in different groups were analyzed statistically. Results GRIM-19 expressed in every stage of preimplantation embryo of mice. The results of real-time PCR showed a gradually increase of GRIM-19 expression from 2-cell to 8-cell, and the expression then decreased progressively. In 8-cell embryo, mRNA expression of GRIM-19 in good quality embryos was significantly higher than that in bad quality embryos (P <0.05).Conclusion The expression of GRIM-19 in mouse preimplantation embryos is changed with the progress in development. GRIM-19 may reflect the potentiality of embryo development.

Key words: Gene associated with retinoid-interferon-induced mortality-19, Preimplantation embryo, Mitochondria, Real-time PCR, Mouse