解剖学报

›› 2012, Vol. 43 ›› Issue (3): 322-327.doi: 10.3969/j.issn.0529-1356.2012.03.007

• 神经生物学 • 上一篇    下一篇

Ghrelin对小鼠空间记忆和海马神经元突触及生长激素促分泌素受体表达的影响

王俊波1; 董静1; 徐麟浩2; 张大勇1; 袁张根1; 凌树才2; 倪晶晶3*   

  1. 1.浙江大学城市学院医学与生命科学学院临床医学系，杭州 310015;2.浙江大学医学院人体解剖学与组织学胚胎学系，杭州 310058;3.宁波天一职业技术学院医学技术学院人体形态学教研室，浙江 宁波 315100
  • 收稿日期:2012-01-19 修回日期:2012-02-15 出版日期:2012-06-06
  • 通讯作者: 倪晶晶

Effects of Ghrelin on spatial memory, hippocampal neuron synapses and growth hormone secretagogue receptor expression in mice

  1. 1.Department of Clinical Medicine, School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou 310015, China; 2.Department of Human Anatomy and Histoembryology, Medical College of Zhejiang University, Hangzhou 310058, China; 3.Departmant of Human Morphology, School of Medical Technology, Ningbo Tianyi Vocational Technology College, Zhejiang Ningbo 315100, China
  • Received:2012-01-19 Revised:2012-02-15 Online:2012-06-06
  • Contact: NI Jing-jing3

关键词: Ghrelin, 学习记忆, 海马, 生长激素促分泌素受体, 突触, Morris水迷宫, 免疫组织化学, 小鼠

Abstract: Objective To investigate effects of Ghrelin on learning and memory processes, hippocampal neuron synapses and growth hormone secretagogue receptor (GHS-R) expression in mice. Methods Twenty male mice (8 weeks old) were randomly divided into the Ghrelin-treated and control groups (10 mice/group) in which Ghrelin or equal quantity saline was intraperitoneally injected, respectively. The ability of spatial learning and memory were tested with Morris water maze (MWM), expression of Ghrelin receptor GHS-R in hippocampus with immunohistochemical method and image analysis, and structural changes of synapsis in the hippocampal region by transmission electron microscope.Results In the MWM experiment, compared with the control group, the escape latency of the treated group on the day 2, 3 and 4, was significantly shortened, the times crossing hidden platform markedly increased (EM>P/EM> <0.05),expression of GHS-R immunoreactive products increased obviously in hippocampus (EM>P/EM> <0.05),and numbers of hippocampal neuron synapses in the CA3 region were also strikingly increased in the Ghrelin-injected group compared with the control group (EM>P/EM> <0.05).Conclusion Ghrelin may increase the synapse numbers of the hippocampal region, by binding the GHS-R in hippocampus, and subsequently enhance spatial learning and memory

Key words: Ghrelin, Learning and memory, Hippocampus, Growth hormone secretagogue receptor, Synapse, Morris water maze, Immunohistochemistry, Mouse

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