›› 2006, Vol. 37 ›› Issue (6): 715-719.doi:
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曾彬;林国生;蔡军
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关键词: END-2细胞, 胚胎干细胞, 心肌细胞, 分化, 共培养, BR>免疫荧光技术, 小鼠 BR>
Abstract: Objective To study the differentiation of embryonic stem cells(ESCs) induced by transforming growth factor-β1(TGF-β1) and co-cultured with visceral endoderm like END-2 cells,and explore cardiomyocytes induction effects of the combined techniques Methods Day 2-3 embryoid bodies (EBs) were derived from ESCs,and then TGF-β1 was added or/and co-cultured with END-2 cells or END-2 cells conditioned medium.Spontaneous differentiation was as a control.The expression of cardiac specific α-sarcmeric actin(α-actin) and cardiac troponin-T(TnT) was detected by immunofluoresence staining.The ultrastructural analysis for ESCs-derived cardiomyocytes was scanned by transmission electron micrograph. Results The total percentage of beating EBs treated with TGF-β1, co-cultured with END-2 cells,or END-2 cell conditioned medium was(43±2.08)%,(69±3.61)%,(65±3.06)%,respectively.All the beating cardiomyocytes derived from ESCs expressed cardiac-specific proteins for α-actin and TnT,and could be observed the cardiac-specific ultrastructure.Interestingly,the total percentage of beating EBs treated with the combined method was (91±1.52)%.(P<0.01),and the beating areas were bigger,and more
Key words: END-2 cell, Embryonic stem cells, Cardiomyocytes, Differentiation, Co-culture, Immunofluoresence staining, Mice
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Q813.1
曾彬;林国生;蔡军. 转化生长因子β-1联合内脏内胚层样END-2细胞共培养对小鼠胚胎干细胞体外分化为心肌细胞的实验研究[J]. , 2006, 37(6): 715-719.
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https://jpxb.bjmu.edu.cn/CN/Y2006/V37/I6/715