解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 224-228.doi: 10.3969/j.issn.0529-1356.2013.02.015

• 肿瘤生物学 • 上一篇    下一篇

乙醛脱氢酶1和雌激素受体在乳腺癌原发灶与复发转移灶之间的表达差异

邵军1  潘翠萍1  王明伟2  吴新红1  马彪1*   

  1.  1.   湖北省肿瘤医院乳腺科; 2. 病理科，武汉 430079
  • 收稿日期:2012-07-06 修回日期:2012-09-02 出版日期:2013-04-06 发布日期:2013-04-06
  • 通讯作者: 邵军 E-mail:shaojun_x@sohu.com
  • 基金资助:

    湖北省卫生厅重点项目;国家杰出青年科学资助项目

Discordance of aldehyde dehydrogenase 1 and estrogen Receptor expression between primary and metastatic focuses of breast cancer

SHAO Jun1 PAN Cui-ping1 WANG Ming-wei 2 WU Xin-hong1 MA Biao 1*    

  1. 1.   Department of Breast Cancer, Hubei Cancer Hospital, Wuhan 430079, China;2. Department of Pathology, Hubei Cancer Hospital, Wuhan 430079, China)
  • Received:2012-07-06 Revised:2012-09-02 Online:2013-04-06 Published:2013-04-06

摘要：

目的 回顾性分析乙醛脱氢酶1（ALDH1）和雌激素受体（ER）在乳腺癌原发灶和对应复发转移灶之间的表达变化。方法 87例复发转移性乳腺癌，免疫组织化学方法检测复发转移灶及其对应的原发灶中ALDH1和ER的表达差异，利用图像分析软件Image-Pro Plus 6.0 (IPP 6.0)对免疫组织化学结果进行积分吸光度（IA）值测定，并进行统计学分析；结合临床病理特征进行相关性分析。 结果 ALDH1在乳腺癌原发灶的阳性率为28.7%（25例），复发转移灶的表达率为43.7%（38例），复发转移灶的阳性率明显高于原发灶，差异有显著性（P <0.05）。ER在原发灶的阳性率为56.3%，在复发转移灶为32.2%，差异有显著性。复发转移灶与对应原发灶IA 值统计分析结果显示，ALDH1的表达量明显升高（P <0.05）,而ER的表达量显著降低（ P <0.01）。ALDH1阳性表达与肿块大小（>2cm）、高组织学分级、淋巴结转移及ER阴性相关。结论 乳腺癌复发转移灶中ALDH1的表达率明显高于原发灶，而ER阳性率要显著低于原发灶。

关键词: 乳腺癌 ,  转移 , 干细胞 ,  乙醛脱氢酶1 ,  雌激素受体 ,  免疫组织化学 , 人

Abstract:

Objective To analyze the difference of aldehyde dehydrogenase 1(ALDH1) and estrogen receptor (ER) expression between primary and corresponding metastatic focuses of breast cancer. Methods A total of 87 female patients diagnosed with metastatic breast cancer were included in this study. Immunohistochemistry (IHC) was used to detect the expression of ALDH1 and ER between primary and metastatic sites. Integrated absorbance (IA) of stained sections was measured by Image-Pro Plus 6.0 (IPP 6.0)，and then the statistical analysis was done. Results The incidences of ALDH1 and ER expression among the primary tumor were 28.7%(25 cases) and 56.3% (49 cases), while ascending to 43.7% and declined to 32.2% in corresponding metastatic breast cancer, which were statistically significant respectively (P <0.05 and P <0.01). Statistical analysis results of IA showed that ALDH1 expression in metastatic focuses was obviously higher than that in the corresponding primary tumor, while ER expression was significantly lower. Conclusion The frequencies of ALDH1 positive expression and ER negative expression were more frequently observed in metastatic focuses than in primary breast cancer.

Key words: Breast cancer , Metastasis , Stem cell , Aldehyde dehydrogenase 1 , Estrogen receptor , Immunohistochemistry , Human