活细胞计数试剂盒在检测5-氮杂-2’-脱氧胞苷对慢性粒细胞白血病细胞的增殖抑制作用中的应用

doi:10.3969/j.issn.0529-1356.2014.04.026

解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 582-584.doi: 10.3969/j.issn.0529-1356.2014.04.026

• 技术方法 • 上一篇

活细胞计数试剂盒在检测5-氮杂-2’-脱氧胞苷对慢性粒细胞白血病细胞的增殖抑制作用中的应用

刘玥¹ 帅春² 李杰生³ 尹虹¹ 宋艳斌¹马文丽^1*

1. 南方医科大学基因工程研究所，广州 510515; 2. 广东省妇幼保健院新生儿科越秀院区，广州 510000;3. 广州军区空军司令部门诊部，广州 510000

收稿日期:2014-03-07 修回日期:2013-11-04 出版日期:2014-08-06 发布日期:2014-08-06
通讯作者: 马文丽 E-mail:yue.yue.l@gmail.com
基金资助:
广东省医学科研基金;南方医科大学2012年度“科研启动计划”青年科技人员培育项目;广东省科技计划项目;广东省自然基金项目

Application of Cell Counting Kit-8 in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells

LIU Yue¹ SHUAI Chun² LI Jie-sheng³YIN Hong¹SONG Yan-bin¹MA Wen-li ^1*

1. Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515，China;2. Department of Neonatology, Yuexiu Branch of Guangdong Maternal and Child Health Hospital, Guangzhou 510000，China;3. Outpatient Department of Guangzhou Military Region Air Force Command, Guangzhou 510000, China

Received:2014-03-07 Revised:2013-11-04 Online:2014-08-06 Published:2014-08-06
Contact: MA Wen-li E-mail:yue.yue.l@gmail.com

摘要/Abstract

摘要：

目的探讨活细胞计数试剂盒（CCK-8）在检测5-氮杂-2’-脱氧胞苷（5-Aza-2’-dc）对慢性粒细胞白血病细胞的增殖抑制作用中的应用。方法采用CCK-8检测不同浓度5-Aza-2’-dc对K562细胞的增殖抑制作用，并检测其半数抑制浓度对K562细胞的细胞周期和凋亡的影响。结果 5-Aza-2’-dc的半数抑制浓度为15.55nmol/L，采用该浓度处理K562细胞后，G2期发生阻滞，增殖抑制，并出现凋亡峰。结论不同浓度的5-Aza-2’-dc对K562细胞增殖的抑制作用不同，且呈浓度依赖关系。CCK-8检测法可运用于甲基化转移酶抑制剂对人慢性粒细胞白血病细胞增殖的影响。

关键词: 活细胞计数试剂盒, 5-氮杂-2’-脱氧胞苷, K562, 增殖

Abstract:

Objective To study the application of Cell Counting Kit-8(CCK-8) in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cell.
Methods The proliferation of K562 cells was detected by CCK-8 with different concentrations of 5-Aza-2’-deoxycytidine and the cell cycle and apoptosis of K562 cells were detected after K562 treated by 50% inhibitory concentration of 5-Aza-2’-deoxycytidine. Results The 50% inhibitory concentration of 5-Aza-2’-deoxycytidine was 15.55nmol/L, after treated with this concentration, K562 cells showed that G2 phase arrest occurred, proliferation inhibited and apoptosis peaks appeared. Conclusion Inhibition of proliferation of K562 cells with different concentrations of 5-Aza-2’-deoxycytidine varied in a dose-dependent relationship, and 5-Aza-2’-deoxycytidine could promote apoptosis of K562 cells. CCK-8 can be used in detecting the effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells.

Key words: Cell Counting Kit-8, 5-Aza-2’-deoxycytidine, K562, Proliferation

刘玥帅春李杰生尹虹宋艳斌马文丽*. 活细胞计数试剂盒在检测5-氮杂-2’-脱氧胞苷对慢性粒细胞白血病细胞的增殖抑制作用中的应用[J]. 解剖学报, 2014, 45(4): 582-584.

LIU Yue SHUAI Chun LI Jie-sheng YIN Hong SONG Yan-bin MA Wen-li*. Application of Cell Counting Kit-8 in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells[J]. AAS, 2014, 45(4): 582-584.

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活细胞计数试剂盒在检测5-氮杂-2’-脱氧胞苷对慢性粒细胞白血病细胞的增殖抑制作用中的应用

Application of Cell Counting Kit-8 in detecting the growth inhibiting effect of 5-Aza-2’-deoxycytidine on chronic myeloid leukemia cells

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