三七总皂苷对脂多糖诱导小鼠抑郁样行为及脑内小胶质细胞表达的影响

doi:10.16098/j.issn.0529-1356.2018.02.005

摘要/Abstract

摘要：

目的探讨三七总皂苷(PNS)对脂多糖(LPS)诱导的小鼠抑郁样行为及脑内小胶质细胞活化的影响。方法 24只雄性昆明小鼠随机分为溶媒对照（NS）组、LPS组、三七总皂苷预处理（PNS+LPS）组，采用新奇环境抑制摄食实验、糖水偏好实验、悬尾实验、强迫游泳实验观察小鼠的抑郁样行为；采用免疫组织化学法检测海马、杏仁核小胶质细胞标记物离子钙接头蛋白分子1（IBA-1）的表达变化。结果 LPS导致小鼠出现抑郁样行为，包括新奇环境摄食潜伏期延长（P<0.05），糖水偏好百分比下降（P<0.05），悬尾不动时间延长（P<0.05），强迫游泳不动时间增加（P<0.01），PNS干预可逆转上述抑郁行为表现（P<0.05）； LPS导致小鼠海马和杏仁核IBA-1阳性细胞数目显著增多（P<0.0001），PNS预处理能明显减少IBA-1的阳性细胞数（P<0.05）。结论三七总皂苷能有效缓解LPS诱导的小鼠抑郁行为，其机制可能与抑制脑内小胶质细胞活化有关。

关键词: 三七总皂苷, 脂多糖, 抑郁, 炎症, 小胶质细胞, 免疫组织化学, 小鼠

Abstract:

Objective To investigate the effect of Panax notoginseng total saponins (PNS) on lipopolysaccharide (LPS)-induced depression-like behavior and expression of microglia in mice. Methods Totally 24 male Kunming mice were randomly divided into NS group, LPS group and PNS+LPS group. Depression-like behavior was investigated by novelty-suppressed feeding test, sucrose preference test, tail suspension test and forced swimming test. Expression of microglia marker ionized calcium binding adaptor molecule-1 (IBA-1) in the hippocampus and amygdala was determined by Immunohistochemistry assess. Results LPS led to prolonged latency in the novelty-suppressed feeding test (P<0.05), reduced sucrose preference (P<0.05), and increased immobility time in the tail suspension test (P<0.05) and forced swimming test (P<0.01), while PNS treatment could effectively reverse the depression-like behavior induced by LPS injection (P<0.05). LPS injection led to significantly increased number of IBA1 positive cells in the hippocampus and amygdala (P<0.0001), which was attenuated by PNS treatment (P<0.05).Conclusion Our findings indicate that PNS can alleviate LPS-induced depression-like behavior in mice, which may be attributed to the inhibiting of microglia activation in the hippocampus and amygdala.

Key words: Panax notoginseng total saponins, Lipopolysaccharide, Depression, Inflammatory, Microglia, Immunohistochemistry, Mouse

曾佳玉王贞牛磊罗诗诗刘政海曹文宇徐杨万炜陈熙. 三七总皂苷对脂多糖诱导小鼠抑郁样行为及脑内小胶质细胞表达的影响[J]. 解剖学报, 2018, 49(2): 166-171.

ZENG Jia-yu WANG Zhen NIU Lei LUO Shi-shi LIU Zheng-hai CAO Wen-yu XU Yang WAN Wei CHEN Xi. Effect of Panax notoginseng total saponin on lipopolysaccharide induced depression-like behavior and expression of microglia in mice[J]. Acta Anatomica Sinica, 2018, 49(2): 166-171.

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