解剖学报

›› 2009, Vol. 40 ›› Issue (3): 507-509.doi: 10.3969/j.issn.0529-1356.2009.03.033

• 论著 • 上一篇    下一篇

成熟生物膜与白假丝酵母菌耐药性相关

李坚1 ;王艾琳1*; 宿丽娟2   

  1. 1.北华大学检验学院， 吉林 132013;2. 吉林化工二院， 吉林 132011
  • 收稿日期:2008-12-15 修回日期:2008-12-24 出版日期:2009-06-06
  • 通讯作者: 王艾琳

Biofilm maturation is correlated to drug resistance of EM>Candida albicans/EM>

  1. 1.Examine College of Beihua University,Jilin 132013,China;2.the Second Chemical Industry Hospital of Jilin,Jilin 132011,China
  • Received:2008-12-15 Revised:2008-12-24 Online:2009-06-06
  • Contact: WANG Ai-lin1

关键词: 生物膜, 耐药性, NCCLS微量稀释法, XTT减低法, 白假丝酵母菌

Abstract: ［Abstract］ Objective To observe the drug resistance at different stages of EM>Candida(C.) albicans /EM>biofilm formation. Methods To form the biofilm in glass slide, then observe the biofilm morphology by inverted microscope at different development stages(2, 4， 8, 24, 48 hours).Using National Committee for Clinical Laboratory Standards(NCCLs) microdilution to detect drug resistance of dissociative EM>C.albicans/EM> and 2,3 bis(2methoxy 4 nitro 5 sulfophenyl) 5(phenylamino) carbonyl 2H tetrazolium hydroxide(XTT) reduction method to test the change of EM>C. albicans/EM> drug resistance in biofilm. Results Antifungal resistance of biofilmgrown cells increased as the biofilm maturation. Compared with dissociative C.albicans strains, the drug resistance was stronger more than 100 times. Conclusion There is positive cor

Key words: Biofilm, Drug resistance, NCCLs microdilution, XTT reduction method, EM>C. albicans/EM>

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