关键词: 肝再生, Genome 230 2.0芯片, 基因表达谱分析, 凝血反应, 免疫磁珠分选, 大鼠

Abstract: Objective To study the transcript atlas of cell immunity-associated genes in 8 liver cell types including hepatocytes, bile duct epithelial cells, oval cells, hepatic stellate cells, sinus endothelial cells, Kupffer cells, pit cells and dendritic cells from the rat regenerating liver. Methods Isolation of rat 8 liver cell types, detection of their genes expression change and the prediction physiological activities were accorded to cluster program, and the methods of bioinformation and systematic biology, and their gene expression patterns were analyzed by Microsoft Excel software. Results A total of 40 blood coagulation-associated genes yielded the meaningful expression changes in liver regeneration. serpine1 , a2m in eight liver cell types, vwf , klkb1 in seven liver cell types other than Kupffer cells, and other genes in the two or more liver cell types yielded the meaningful expression changes. It suggested that the synthesis of kallikrein and prothrombin、the formation of thrombin were enhanced at the priming and progressing phases of liver regeneration. The biological activities, such as fibrin monomer aggregated into fibrin polymers, were elevated at termination phase. Conclusion The rat liver regeneration is closely associated with the blood coagulation.

Key words: Liver regeneration, Genome 230 2.0 array, Gene transcription profiles, Blood coagulation, Immune magnetic beads separation, Rat