关键词: 雷帕霉素, 环孢霉素A, 紫杉醇, 巨噬细胞, 流式细胞术, 小鼠

Abstract: Objective To investigate the effects of three immunospressive drugs rapamycin (rapa) and cyclosporin A (CsA) and paclitaxel on the phenotypes and functions of macrophages differentiated from bone marrow progenitor cells (BMPCs). BR> Methods BMPCs were prepared by aseptic technique from 60 C57BL/6 mice killed by cervical dislocation and 〖WTBX〗in vitro cultured in media containing rapa (100μmol/L), CsA (1mg/L) and paclitaxel（20μg/L）respectively together with macrophage colony-stimulating factor (M-CSF). Phenotypes and functions of differentiated macrophages were analyzed by mixed lymphocyte reaction (MLR) and flow cytometry (FCM). Results There were significantly decreased expressions of surface molecule CD80 and CD54 in differentated macrophages treated with rapa, and a significant decrease expression in CD80, CD40 and I-ASUP>d/SUP> of macrophages treated with CsA. The expressions of CD80, CD86 and CD14 were of significant increase and a significantly decreased I-ASUP>d/SUP> expression in differentated macrophages treated with paclitaxel.Compared with control group, there was a decaese in phagocytic and antigen presenting ability of induced macrophages treated with CsA and rapa, however, macrophages treated with paciltaxel showed significantly strong phagocytosis and section IL-12, as well as significantly reduced DTH reaction.Conclusion The experimental results indicate that three immunosuppressive drugs may affect phenot

Key words: Rapamycin, Cyclosporin A, Paclitaxel, Macrophage, Flow cytometry, Mouse