解剖学报

›› 2011, Vol. 42 ›› Issue (2): 283-287.doi: 10.3969/j.issn.0529-1356.2011.02.029

• 论著 • 上一篇    

层黏连蛋白受体在小鼠睾丸和附睾中的表达

李善妮1;王行明1;孙双午1;王璐1;黄霁2;滕春波3;丁乃峥1*   

  1. 1.中南大学生物科学与技术学院,长沙 410013;2.湖南师范大学树达学院,长沙 410081;3.东北林业大学生物科学学院,哈尔滨 150040
  • 收稿日期:2010-04-12 修回日期:2010-05-26 出版日期:2011-04-06
  • 通讯作者: 丁乃峥

Expression of laminin receptor in testis and epididymis of adult male mice

  1. 1.School of Biological Science and Technology,Central South University, Changsha410013,China;2.Shuda College, Hu’nan Normal University, Changsha410081,China;3.College of Life Science, Northeast Forestry University, Harbin150040, China
  • Received:2010-04-12 Revised:2010-05-26 Online:2011-04-06
  • Contact: DING Nai-zheng

关键词: 层黏连蛋白受体, 睾丸, 附睾, 原位杂交, 免疫组织化学, 小鼠

Abstract: Objective To provide a basis of further studying functional significance of the laminin receptor 1 (LAMR1) in spermatogenesis, sperm mature and fertilization, the expression of the LAMR1 in testis and epididymis of adult male mice was studied. Methods Testis and epididymis from three normal Kunming white male mice were collected. EM>In situ/EM> hybridization and immunohistochemistry were applied to detect the expression of the LAMR1 in testis and epididymis of adult male mice. Results The result of in situ hybridization showed that LAMR1 mRNA expressed in the epididymis caput and cauda at high levels，and also was detected in spermatogenic cell and spermatids. The immunohistochemistry result showed that the intensities of immunohistochemical staining of LAMR1 tended to strengthen from epididymal caput to epididymal cauda, and also was detected in spermatogenic cells, spermatids and epididymis spermatozoa. Conclusion LAMR1 has a specific distribution on testis and epididymis of adult mice, suggesting that it is likely to have some relations to the spermatogenesis and function of epididymis. The high expression in epididymis cauda spermatozoa supports the hypothesis that LAMR1 plays a role during fertilization.

Key words: Laminin receptor, Testis, Epididymis, In situ hybridization, Immunohistochemistry, Mouse

中图分类号: