Division mode of cultured rat cardiac telocytes

doi:10.16098/j.issn.0529-1356.2015.05.009

Abstract

Abstract:

Objective To explore the Objective To explore the division mode of rat cardiac telocytes in vitroand to provide the morphological evidence for further research of telocytes. Methods Cardiac telocytes were isolated from neonatal rats and cultured with a combined type I collagenase(W/V, 0.1%) -trypsin (W/V, 0.125%) digestion method. The dynamic process of telocytes division was captured under a Live Cell Imaging System or with hematoxylin staining. Results Telocytes cultured with combined enzymes digestion were of high purity, survival for long time and rapid proliferation. Amitosis was observed in telocytes which has a large and budded nuclei. The dumbbell nucleus was then elongated and divided between the mother and daughter cells within the bud neck. Mitosis was characterized by the congregated chromosomes which were gradually shortened and coarsened, and nuclear chromatin arranged tidily in the equatorial plane during prometaphase. Chrosomes were migrated to opposite poles and eventually the formation of the two daughter nuclei was completed during telophase. During cytokinesis, cytoplasm was pulled apart gradually and two new individual daughter cells came into being. Telocytes maintained their typical morphological features in different phases of mitosis. Conclusion There are two different division types in cardiac telocytes in vitro: namely amitosis and mitosis.

Key words: Amitosis, Mitosis, Living cell station, Telocytes, Enzyme digestion method, Neonatal rat

CHANG Yu-qiao LI Ci-xia GUO Zhi-kun* LI He*GAN Li NIU Yan-fei. Division mode of cultured rat cardiac telocytes[J]. AAS, 2015, 46(5): 629-633.

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