P2X3 mRNA expression in L4-6 dorsal root ganglion at different times after the spared nerve injury

doi:10.16098/j.issn.0529-1356.2016.03.002

Abstract

Abstract:

Objective To observe the mRNA expression of P2X3 in L4-6 dorsal root ganglion (DRG) at different times after spared nerve injury (SNI), and to discuss the effect of periphery P2X3 in neuropathic pain. Methods Fifty-four healthy male SD rats were randomly divided into control group, sham surgery group, and surgery group. The spared nerve injury model was established by ligating the common peroneal and the tibial nerves and then cutting off the nerves but keeping the sural nerve intact. Bilateral paw withdrawal thresholds were dynamic observed before injury (base), at day 1, 3, 7 and day 14 after injury. P2X3 mRNA expression in L4-6 DRG at day 3, 7, and day 14 after injury was measured by fluorescence quantitative PCR method. Results Compared with control group, ispilateral paw withdrawal thresholds of surgery group were significantly decreased at day 1, 3, 7, and day 14 after injury (P<0.05), while that of sham surgery had no statistical significance (P>0.05). There was no difference of contralateral pain threshold among control group, surgery group, and sham surgery group (P>0.05). P2X3 mRNA expressions of L4, L5, and L6 DRG of surgery group were remarkable increased at day 3, 7 after injury (P<0.05), while that of L5, L6 DRG were decreased at 14d after injury (P<0.05), and that of L4 DRG were more than that of sham surgery at day 14 after injury (P<0.05). In addition, there was no difference of P2X3 mRNA expression in L4, L5, and L6 DRG at every time after injury between sham surgery group and surgery group. Conclusion Periphery P2X3 mRNA expression is involved in the initiation and maintenance of pain induced by SNI, which may play a different effect at different phases.

DU Jun-ying FANG Jun-fan FANG Jian-qiao* LE Xiao-qin XIAO Ting PAN Ning-fang YU Jie. P2X3 mRNA expression in L4-6 dorsal root ganglion at different times after the spared nerve injury[J]. AAS, 2016, (3): 297-302.

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