AAS ›› 2016, Vol. ›› Issue (3): 330-336.doi: 10.16098/j.issn.0529-1356.2016.03.007

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Protective effects of ethyl acetate extract from Chroogomphis rutilus on SH-SY5Y cells damaged by 1-methyl-4-phenyl pyridine ion

LI Ge YAO Hui LIU Chuan GAO Zhi-guo WANG Li-an GUAN Zhen-long WANG Yan-qin*   

  1. College of Life Science, Hebei Normal University, Shijiazhuang 050024, China
  • Received:2015-09-15 Revised:2016-02-23 Online:2016-06-06 Published:2016-06-06
  • Supported by:

    Natural Science Foundation of Hebei Province of China

Abstract:

Objective The aim of present study is to observe the protective effects of ethyl acetate extract fromChroogomphis rutiluson dopaminergic neurons damaged by 1-methyl-4-phenyl pyridine ion (MPP+). Methods SH-SY5Y cells (dopaminergic cells), a cell line of human neuroblastoma, were served as the experimental subject. We examined the IC50 of MPP+ at 48 hours. The cells were preincubated with ethyl acetate extract fromChroogomphis rutilusfor 4 hours with 25mg/L, 50mg/L, 100mg/L respectively, then added MPP+ 1.0mmol/L(IC50 value) to incubate for 48 hours. MTT assay, Hoechst 33342 staining and flow cytometric assay were used to detect the survival or apoptosis of SH-SY5Y cells. DCFH-DA was used to detect the reactive oxygen speciess (ROS) level on the cells of ethyl acetate extract fromChroogomphis rutilus. Results 1.0mmol/L MPP+ caused the IC50 of cells at 48 hour, so 1.0mmol/L was used as the lesion concentration. Pretreatment with ethyl acetate extract fromChroogomphis rutilus (50mg/L) significantly increased the cell viability, and reduced the apoptosis rate of cells. After MPP+ lesion,the ROS level increased significantly, and the preincubation with ethyl acetate extract fromChroogomphis rutilus reduced the ROS level induced by MPP+. Conclusion Ethyl acetate extract from Chroogomphis rutilus can significantly protect dopaminergic cells damaged with MPP+.