AAS ›› 2016, Vol. ›› Issue (3): 353-358.doi: 10.16098/j.issn.0529-1356.2016.03.011

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Sodium selenite induces apoptosis of human gastric cancer SGC-7901 cells through mitochondrial-dependent pathway

WANG Yan 1,2 ZHAO Shang1 SU Yan-ping 1* LIU Li-wei1 WANG Hui1 QU Peng 1*   

  1. 1. Departmen of Histology and Embryology, Taishan Medical College, Shandong Taian 271000, China;2. Departmen of Anatomy, Weifang Career Academy of Nursing, Sahndong Qingzhou 262500, China
  • Received:2015-09-10 Revised:2016-02-03 Online:2016-06-06 Published:2016-06-06
  • Contact: SU Yan-ping;QU Peng E-mail:su-yanping@163.com

Abstract:

Objective To study the mechanism of gastric cancer cells apoptosis induced by sodium selenite,by investigating the expression of Bax/Bcl-2, the change of mitochondrion membrane potential and cytochrome C(Cyt C) in the process apoptosis ofof human gastric cancer SGC-7901 cells. Methods Human gastric cancer SGC-7901 cell line was added different concentrations (2.5, 5.0, 10.0μmol/L) of sodium seleite for 24hours,48hours. The expression of Bax/Bcl-2 protein was detected by immunocytochemical and Western blotting methods. SGC-7901 cells were stained by Rhodamine123 to detect changes of mitochondrion membrane potential by flow cytometry. The changes of cytoplasm Cyt C and mitochondria Cyt C protein content were detected by Western blotting. Results Immunocytochemistry and Western blotting showed that sodium selenite increased the Bax protein levels(P<0.05) and reduced the Bcl-2 protein levels (P<0.05) in dose-dependent manner.Flow cytometry technique results showed that sodium selenite reduced mitochondrial membrane potential(P<0.05). The results indicated that sodium selenite may induce SGC-7901 cells apoptosis by altering Bax/Bcl-2 and reducing mitochondrial membrane potential. Western blotting showed that sodium selenite increased the cytoplasm Cyt C protein levels(P<0.05) and reduced the mitochondria Cyt C protein levels (P<0.05), therefore promoted the release of Cyt C to cytoplasm. Conclusion Sodium selenite can induce apoptosis of human gastric cancer SGC-7901 cells by increasing Bax and discreasing Bcl-2 gene expression, reduce the mitochondrial membrane potential, to promote Cyt C release from mitochondria to the cytoplasm, and induce human gastric cancer SGC-7901 cell apoptosis through mitochondrial-dependent pathway.