Effects of the ephedrine injection followed by a stomach irrigation of Chinese medicine compound in the pregnant mouse on hepatic tissue structure and antioxidant enzymes activity of the filial mice

doi:10.16098/j.issn.0529-1356.2016.03.014

Abstract

Abstract:

Objective To explore effects of ephedrine injection followed by the stomach filling of Chinese medicine compound in the pregnant mouse on the development of the filial mice. Methods Totally 30 pregnant mice were used in the present study and divided into 3 groups，the natural control group，experimental control group and experimental group.The pregnant mice of experimental control group and experimental group were continuously intraperitoneally injected 6.0 g/L ephedrine (twice a day， 0.2ml each)，the natural control group was injected with the same amount of saline.The pregnant mice of experimental group were irrigated to stomach with 30.0 g/L Chinese medicine compound 0.2ml 1 hour after ephedrine injection.The natural control group and experimental control group were irrigated to stomach with same amount of saline.On day 5，10 and 15 after delivery，the activities of filial mice hepatic histological total antioxidant capacity（T-AOC），glutathione S-transferase（GST）and plasma alanine aminotransferase（ALT)，as well as content of malondialdehyde（MDA）MDA were determined by colorimetry，and the liver tissue structures of filial mice were observed by optical microscopy，the expression changes of TGF-β1 protein were measured by immunohistochemical staining. Results Compared with natural control group the activity of filial mice’s hepatic histological T-AOC，and GST of experimental control group were significantly reduced，while the content of MDA was increased significantly，the activity of plasma ALT was significantly increased，the filial mice’s liver tissue appeared varying degrees of damage，the expression intensity of TGF-β1 protein in liver tissue was significantly increased (P<0.05 or P<0.01).But the activity of T-AOC，GST of the filial mice’s liver tissue in the experimental group were increased significantly，the content of MDA was significantly decreased，and the activity of plasma ALT was significantly reduced.The pathological damage of liver was significantly improved，the expression of TGF-β1 protein was decreased (P<0.05 or P<0.01). Conclusion Ephedrine affects the histological structure and antioxidant enzymes activity and TGF-β1 expression of the liver.The female mice with stomach irrigation of Chinese medicine compound may enhance filial mice liver cells antioxidant enzymes activity，reduce TGF-β1 protein expression of liver tissue，and lighten the damage of filial mice liver tissue by ephedrine injecting of female mice.

LI Chong-yang YANG Ya-fei ZHAO Wen-cheng YU Shi-yuan*. Effects of the ephedrine injection followed by a stomach irrigation of Chinese medicine compound in the pregnant mouse on hepatic tissue structure and antioxidant enzymes activity of the filial mice[J]. AAS, 2016, (3): 368-373.

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