Impacts of RNAi silencing on the CD105 and Ki67 gene expression in ovarian cancer OVCAR3 cell lines

doi:10.16098/j.issn.0529-1356.2016.04.011

Abstract

Abstract:

Objective To detect changes of the CD105 and Ki67 gene expression and to determine the effect of gene silence on OVCARs cells transfected these gene sequences by liposome lipofectamine TM2000 respectively. Methods OVCAR3 were transfected with siRNA of CD105-siRNA group, Ki67-siRNA group and negative control groups in vitro respectively. The expressions of CD105mRNA, Ki67mRNA and their proteins were detected with Real-time PCR and Western blotting. Results The intensities of relative expressions for both CD105 mRNA and Ki 67mRNA were significantly lower in both siRNA-CD105 transfected and siRNA-Ki67-transfected OVCARs than those in control groups(P<0.001). Conclusion CD105-siRNA and Ki67-siRNA can specifically inhibit CD105, Ki67 gene expression levels in ovarian cancer OVCAR3 cells and lowered their mRNA and protein expression.

Key words: CD105, Ki67, Real-time PCR, Western blotting, Human

GUO Hai-rong WANG Xiao-yan HE Shuai LIU Ping*. Impacts of RNAi silencing on the CD105 and Ki67 gene expression in ovarian cancer OVCAR3 cell lines[J]. AAS, 2016, 47(4): 502-506.

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