Effects of homocysteine on histone modification during oocyte development in mice

doi:10.16098/j.issn.0529-1356.2017.05.015

Abstract

Abstract:

Objective To understand the effect of homocysteine（HCY）on histone modification during oocyte development. Methods In this paper the follicle culture system (10 female ICR mouse of 2 weeks) and method of oocyte maturated in vitro (10 female ICR mouse of 4weeks) were used. The processes of oocyte development and maturation were observed. Immunofluorescent staining was performed to show the influence of HCY on distribution of methylated histone H3K4, H3K9, and acetylated histone H3K9 during early stage of oocyte development. With the approach of Real-time PCR, the expression levels of two enzymes controlling histone acetylation, histone deacetylase(HDAC) and histone acetyltransferase (GCN5), in oocyte maturated in vitro were compared between the groups of coculture with homocysteine and control. Results The process of oocyte maturation was significantly inhibited by homocysteine. The distribution patterns of methylated histone H3K4, H3K9 and acetylated histone H3K9 and the staining intensity were decreased by homocysteine. The chromatin appeared and slightly decondensated. The influernce of HCY on GCN5 expression was small, but the expression of HDAC in oocyte was significantly inhibited.Conclusion The effect of homocysteine on histone methylation and acetylation is present during oocyte development. This may be the good reason for the decline of nuclear chromosome stability caused by homocysteine.

Key words: Oocyte development, Homocysteine, Histone methylation, Histone acetylation, Follicle culture, In vitro maturation, Mouse

LIANG Rong CHEN Xi ZHANG Yu-jun SHI Cheng. Effects of homocysteine on histone modification during oocyte development in mice[J]. Acta Anatomica Sinica, 2017, 48(5): 585-589.

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