Screening and identification the hub genes of cardiac hypertrophy in mice

doi:10.16098/j.issn.0529-1356.2021.03.010

Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (3): 391-397.doi: 10.16098/j.issn.0529-1356.2021.03.010

• Cell and Molecules Biology • Previous Articles Next Articles

Screening and identification the hub genes of cardiac hypertrophy in mice

SHI Zhuo^1,2,3 LI Jia-hui⁴ GAO Jing⁵ DONG Yan-li⁶ REN Fu^1,2* WEN Pu-shuai^2,7*

1.Department of Anatomy, School of Basic Medical Science, Jinzhou Medical University, Liaoning Jinzhou 121000, China; 2.Liaoning Key Research Laboratory of Human Phenotype Group, Jinzhou Medical University, Liaoning Jinzhou 121000, China; 3.Department of Nursing, Jinzhou Health School, Liaoning Jinzhou 121000, China; 4.The Third Clinical Medical College, Jinzhou Medical University, Liaoning Jinzhou 121000, China; 5.Department of Ultrasound, the First Affiliated Hospital of Jinzhou Medical University, Liaoning Jinzhou 121000, China; 6.Nursing Group of Experimental Center, Chaoyang Health School, Liaoning Chaoyang 122000, China; 7.Department of Pathophysiology, School of Basic Medical Science, Jinzhou Medical University, Liaoning Jinzhou 121000, China

Received:2020-07-14 Revised:2020-09-04 Online:2021-06-06 Published:2021-06-06
Contact: REN Fu;WEN Pu-shuai E-mail:rf@symc.edu.cn
Supported by:
Supported by the Natural Science Foundation of Liaoning Provincial Department of Science and Technology

Abstract

Abstract:

Objective To screen and identify the hub genes closely related to cardiac hypertrophy by using bioinformaticsmethod and biological experiments. Methods The chip data related to cardiac hypertrophy in mice were downloaded from the Gene Expression Omnibus(GEO) database, and the GEO2R online tool was adopted to screen for differentially expressed genes; DAVID 6.7, String 11.0 and Cytoscape 3.7.0 softwares were used to analyze differentially expressed genes; Kunming mice were randomly divided into a normal saline group (n=6) and an angiotensin Ⅱ (Ang Ⅱ) group (n=6) to establish a cardiac hypertrophy model, the expression of hub genes in Kunming mouse model of cardiac hypertrophy induced by AngⅡ was detected by Real-time PCR method . Results A total of 202 common differentially expressed genes and 12 hub genes were selected; the Real-time PCR result demonstrated that decorin(Dcn), HADHA and heat shock protein(HSP)90αA1 were significantly down-regulated in the AngⅡ group. Conclusion The selected hub genes can influence the development of cardiac hypertrophy in Kunming mice through extracellular matrix and transforming growth factor β(TGF-β).

Key words: Cardiac hypertrophy, Bioinformatics, Differentially expressed gene, Real-time PCR, Kunming mouse

CLC Number:

R361

SHI Zhuo LI Jia-hui GAO Jing DONG Yan-li REN Fu WEN Pu-shuai. Screening and identification the hub genes of cardiac hypertrophy in mice[J]. Acta Anatomica Sinica, 2021, 52(3): 391-397.

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Screening and identification the hub genes of cardiac hypertrophy in mice

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