Effects of tricholoma matsutake polysaccharides on
1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage

doi:10.16098/j.issn.0529-1356.2024.01.007

Abstract

Abstract:

Objective To investigate the protective mechanism of tricholoma matsutake polysaccharides（TMP）against 1-methy-4-pehnyl-pyridine ion (MPP⁺)-induced PC12 cell damage. Methods An Parkinson’s disease (PD) PC12 cell model in vitro was constructed using MPP⁺, and PC12 cell were randomly divided into 5 groups: control group（Ctrl）, model group(MDL), TMP high concentration (250 μg/L) group (TMP-H), TMP medium concentration (50 μg/L) group (TMP-M), and TMP low concentration (10 μg/L)group (TMP-L), 3 complex wells in each group. Matsutake polysaccharide treated PC12 cells for 24 hours, 250 μmol/L MPP⁺ induced PC12 cells were prepared for 24 hours to prepare PD cell models. The cell viability of each group of PC12 cell was detected by CCK-8 method, the lactate dehydrogenase (LDH) release of each group of PC12 cell was detected by LDH method, mitochondrial activity was detected by fluorescence microscopy, the average length of mitochondrial network branches and the number of network branches were calculated by Image J 1.53 r software, the mitochondrial membrane potential was detected by JC-1 method, and the protein Bcl-2/Bax ratio was detected by Western blotting method. Results Compared with the Ctrl group, the survival rate of PC12 cell in the MDL group decreased, the LDH release level increased, and the mitochondrial membrane potential (MMP, ΔΨm) and Bcl-2/Bax ratio decreased (all P<0.05). Compared with the MDL group, the cell survival rate of matsutake polysaccharide in the TMP-H group and the TMP-M group increased, the LDH release level decreased, the ΔΨm and Bcl-2/Bax ratio increased (P<0.05), mitochondrial activity increased, the average length of mitochondrial network branches and the number of network branches increased, and the antagonistic membrane potential decreased (P<0.05), among which the matsutake polysaccharide group was more obvious in the reduction of antagonistic membrane potential. Conclusion TMP have a protective effect against MPP⁺-induced PC12 cell damage, possibly by improving cell viability, reducing LDH release, antagonizing the reduction of mitochondrial membrane potential, and repairing mitochondrial network morphology, thereby reducing apoptosis.

Key words: Matsutake polysaccharide, Mitochondrial membrane potential, Parkinson’s disease, PC12 cell, Western blotting

CLC Number:

R285.5，R742.5

LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei .

Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.

References

[1] Kin K, Yasuhara T, Kameda M, et al. Animal models for Parkinson’s disease research: trends in the 2000s ［J］. Int J Mol Sci, 2019, 20 (21): 5402.

［2］Liao JY, Jiang XL, Xie WF. Micro RNA-486 targeting TRIM10 to inhibit Parkinson’s disease cell model damage ［J］. Acta Anatomica Sinica, 2022, 53,(4): 424-431. (in Chinese)

廖建云,江新柳,谢为法.微小RNA-486靶向TRIM10抑制帕金森病细胞模型损伤［J］.解剖学报,2022,53(4):424-431.

［3］Bezard E, Dovero S, Prunier C, et al. Relationship between the appearance of symptoms and the level of nigrostriatal degeneration in a progressive 1-methyL-4-phenyL-1, 2, 3, 6-tetrahydropyridine-lesioned macaque model of Parkinson’s disease ［J］. J Neurosci, 2001, 21(17)：6853-6861.

［4］Wu DC, Teismann P, Tieu K, et al. NADPH oxidase mediates oxidative stress in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson’s disease ［J］. Proc Natl Acad Sci USA, 2003, 100(10): 6145-6150.

［5］Perier C, Bové J, Vila M. Mitochondria and programmed cell death in Parkinson’s disease: apoptosis and beyond ［J］. Antioxid Redox Signal, 2012, 16(9): 883-895.

［6］He YCh, Mao P, Li XM, et al. Active substances and product development status of matsutake mushrooms ［J］. Journal of Microbiology, 2019, 39(2):120-125.(in Chinese)

贺元川,毛萍,李晓明,等.松茸的活性物质及产品开发现状［J］.微生物学杂志,2019,39(2):120-125.

［7］Zhang LW. Study on the application of tricholomamatsutake polysaccharide in relieving Athletes’aerobic exercise fatigue ［J］. Edible fungi of China, 2020, 39(1):49-51. (in Chinese)

张利伟.松茸多糖对缓解运动员有氧运动疲劳恢复应用研究［J］.中国食用菌,2020,39(1):49-51.

［8］Liu L, Yang S, Wang H. α-lipoic acid alleviates ferroptosis in the MPP+ -induced PC12 cells via activating the PI3K/Akt/Nrf2 pathway ［J］. Cell Biol Int, 2021,45(2): 422-431.

［9］Afentou N, Jarl J, Gerdtham UG, et al. Economic evaluation of interventions in Parkinson’s disease: a systematic literature review ［J］. Mov Disord Clin Pract, 2019, 6 (4):282-290.

［10］Yi F, He X, Wang D. Lycopene protects against MPP+ -induced cytotoxicity by maintaining mitochondrial function in SH-SY5Y cells ［J］. Neurochem Res, 2013, 38(8): 1747-1757.

［11］Li TT, Liang YC. In vitro models of Parkinson disease ［J］. Med Recapitulate, 2013, 19(6):1003-1006.

［12］Ma HJ, Gai C, Chai Y, et al. Bu-Yin-Qian-Zheng formula ameliorates MPP+ -induced mitochondrial dysfunction in Parkinson’s disease via parkin ［J］. Front Pharmacol, 2020, 11:577017.

［13］Ding X, Tang J, Cao M, et al. Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Tricholoma matsutake ［J］. Int J Biol Macromol, 2010, 47(2): 271-275.

［14］Fan L, Li ShL, Yin Ch, et al. Effect of low molecular polysaccharide from agaricus blazei on oxidative injury in hippocampal neuronal cells of rats ［J］. Herald of Medicine, 2017, 36(5): 473-476. (in Chinese)

樊丽,李世玲,伊超,等.低分子姬松茸多糖对大鼠海马神经细胞氧化损伤模型的影响［J］.医药导报,2017,36(5):473-476.

［15］Liu J, Yang F, Cheng MF, et al. Spinal cord injury can be relieved by the polysaccharides of tricholoma matsutake by promoting axon regeneration and reducing neuroinflammation ［J］. Neuroreport, 2020, 31(14):1024-1029.

［16］Lei JY, Zhu M. Study on the protective mechanism of matsutake polysaccharide on MPP+ damage PC12 cells ［J］. The World’s Latest Medical Information Digest, 2018, 18(38):39-40, 42.(in Chinese)

雷佳宇,朱梅.松茸多糖对MPP+损伤PC12细胞保护机制的研究［J］.世界最新医学信息文摘,2018,18(38):39-40,42.

［17］Jackson JG, Robinson MB. Regulation of mitochondrial dynamics in astrocytes: mechanisms,consequences, and unknowns ［J］. Glia, 2018, 66(6):1213-1234.

［18］Stojkovska I, Krainc D, Mazzulli JR. Molecular mechanisms of α-synuclein and GBA1 in Parkinson’s disease ［J］. Cell Tissue Res, 2018, 373(1):51-60.

［19］Zhai XH, Lai XH, Wu QH, et al. Effects of coenzyme Q10 on mitochondrial permeability transition of regenerating hepatocytes in rats ［J］. Acta Anatomica Sinica, 2013, 44(1):44-48. (in Chinese)

翟心慧,来小海,吴清华,等.辅酶Q-(10)对大鼠再生肝细胞线粒体通透性转换的影响［J］.解剖学报,2013,44(1):44-48.

［20］Huang QS, LI HZh, Chen AK. Study on mitochondrial pathway of HepG2 cell apoptosis induced by agaricus blazei polysaccharide ［J］. Guangdong Agricultural Sciences, 2013, 40(15): 162-163,175. (in Chinese)

黄清松,李红枝,陈爱葵.姬松茸多糖诱导HepG2细胞凋亡的线粒体通路研究［J］.广东农业科学,2013,40(15):162-163,175.

[1]	LIU Ye CHU Ya-nan XU Ce-lu HE Jia-cheng SU Bing-yin TAI Hao-ran. Roscovitine rescuing neuronal loss and neuroinflammation in brain regions associated with Parkinson’s disease mice [J]. Acta Anatomica Sinica, 2023, 54(6): 635-643.
[2]	YAN Kun WU Xiao-lin LIU Ying-juan GE Ke-li1 REN Lei-ming LI Hong-yun . Effect of cerebrotein hydrolysate-Ⅰon intestinal microflora regulation of mice with Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(5): 497-504.
[3]	LI Meng-yi WU An-ting XU Ze-ting ZHANG Ting LI Jun-wei ZHOU Peng CUI Huai-rui SUN Chen-you. Effect and molecular mechanism of mammalian target of rapamycin complex 2/Akt signaling pathway on 6-hydroxydopamine-treated SH-SY5Y cell model [J]. Acta Anatomica Sinica, 2023, 54(5): 521-530.
[4]	YAN Yang-yang GUO Kai SUN Zhi-hong . Purinergic ligand-gated ion channel 7 receptor-mediated cognitive impairment in Parkinson’s disease rats [J]. Acta Anatomica Sinica, 2023, 54(5): 531-537.
[5]	LIU Ke-ting CHEN Bin-bin SHUI Yu-lian SHI Qing-ming XIAO Li. Neuroprotective effect of exogenous hydrogen sulfide and the expression of matrix metalloproteinases-9 and Caspase-1 in mice model of Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(3): 289-295.
[6]	SUN Meng-fang CHENG Yi-sheng WANG Feng JIN Meng-hao WANG De-xiu. Effect of bergapten on glucose and oxygen deprivation induced PC12 cell injury by regulating long non-coding RNA opioid receptor gene expression [J]. Acta Anatomica Sinica, 2023, 54(1): 56-62.
[7]	BIAN Wei LI Meng-yi ZHOU Peng LI Jun-wei ZHANG Ting WU An-ting QI Shuang-shuang CUI Huai-rui SUN Chen-you. Effects of activating mTORC2/Akt signaling pathway on dopaminergic neurons and behaviors in 6-hydroxydopamin model mice [J]. Acta Anatomica Sinica, 2023, 54(1): 13-22.
[8]	LI Wei WANG Li WANG Zhi-hua LIU Qing-chun HAN Rong-sheng . Protective effect of long non-coding RNA cytoskeleton regulatory RNA targeting microRNA-1246 on cell damage in Parkinson’s disease models [J]. Acta Anatomica Sinica, 2022, 53(5): 563-570.
[9]	LIAO Jian-yun JIANG Xin-liu XIE Wei-fa. MicroRNA-486 targeting TRIM10 to inhibit Parkinson’s disease cell model damage [J]. Acta Anatomica Sinica, 2022, 53(4): 424-431.
[10]	REN Ya-li GUO Lei PAN San-qiang. Amphetamine causing damage of dopamine cells via inhibiting of protein kinase B/glycogen synthase kinase-3β/collapsin response mediator protein-2 signal pathway [J]. Acta Anatomica Sinica, 2021, 52(1): 14-20.
[11]	WANG Tong-tong CHEN Zhi-chi YE Xin BIAN wei DU Juan-juan FU Wei-da CHEN Meng-jiao LI Jun-nan SUN Chen-you. Allopregnanolone restores dopaminergic neurons and motor performance in Parkinson’s disease mice and its molecular mechanisms [J]. Acta Anatomica Sinica, 2020, 51(4): 473-482.
[12]	HAN Qiu-qin WAN Guo-qing GU Xue-feng. Detection of intestinal microbial changes in rotenone-induced Parkinson’s disease model mice#br# [J]. Acta Anatomica Sinica, 2020, 51(4): 507-512.
[13]	ZHANG Xue-ning JIN Xiao-fei ZHOU Xiao-hong GAO Wei-juan. Effects of calycosin on mitochondrial apoptotic pathway in oxygen-glucose deprivation/reoxygenation PC12 cells#br# [J]. Acta Anatomica Sinica, 2020, 51(3): 332-337.
[14]	LIU Gui-xue SHI Yong-hong WANG Shi-qing LI Wen-sheng. Differential analysis of brain structure volume associated with Parkinson’s disease based on nuclear magnetic resonance T1 imaging [J]. Acta Anatomica Sinica, 2018, 49(3): 347-354.
[15]	XIE Ming-qi CHEN Zhi-chi QI Shuang-shuang WANG Tong-tong ZHANG Peng HUANG Hou-ju ZHOU Peng CUI Huai-rui SUN Chen-you. Effects of 6-hydroxydopamine on dopaminergic neurons in nigrostriatal system and on behavior of adult mice [J]. Acta Anatomica Sinica, 2017, 48(4): 361-374.

Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments