Distribution and localization of dopamine receptor in small intestines

doi:10.16098/j.issn.0529-1356.2024.05.013

Abstract

Abstract:

Objective To explore the distribution and localization of dopamine receptor D3-D5 in the small intestine of different species. Methods The distribution and expression of D3-D5 in the small intestine of mice, rats and rhesus monkeys were detected by immunohistochemistry and Western blotting. The expression of D3-D5 in immunoglobulin A positive plasma cells (IgA+PC) located in the lamina propria (LP) were detected by immunofluorescence double labeling. Results D3 and D5 were widely distributed in the epithelium, LP, submucosal plexus (SMP) and intermuscular plexus (MP) of the small intestine in mice, rats and rhesus monkeys. The distribution of D4 in the small intestinal of mice and rhesus monkeys were consistent with the result of D3 and D5. D4 was distributed only within the epithelium and LP of rat small intestine. D3 and D5 were expressed in the IgA+PC in the LP of mice and rats, whereas D4 was not. Conclusion The distribution and localization pattern of D3 and D5 are similar in the small intestine of mice, rats and rhesus monkeys, whereas those of D4 vary between different species. Dopamine may be involved in regulating the functions of IgA+PC.

Key words:

"> Dopamine receptor｜Small intestine｜Immunoglobulin A positive plasma cell｜Western blotting｜Immunofluorescence｜Mouse｜Rat｜Rhesus monkey

CLC Number:

R322.4

CHEN Jun-jun ZHOU Li SU Tian WANG Xian-wei ZHANG Hai-long WANG Zhi-yong. Distribution and localization of dopamine receptor in small intestines[J]. Acta Anatomica Sinica, 2024, 55(5): 612-618.

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