Zdhhc17 regulating convergent and extension movements during zebrafish embryogenesis

doi:10.3969/j.issn.0529-1356.2013.02.022

Abstract

Abstract:

Objective To investigate the role of zdhhc17 on embryonic development in zebrafish by loss-of-function experiments. Methods Zdhhc17 antisense morpholino oligonucleotide was injected into embryos to block the translation. The whole mount in situ hybridyzation and RT-PCR were used to detect the abnormal development after knockdown of zdhhc17. Results Knockdown of zdhhc17 impacted convergence and extension movements during zebrafish gastrulation. The tail of zdhhc17 morphants was curved and shorter (82/118). The expression patterns of dlx3b, ntl, myod1 and tbx6 were affected. The mRNA level of cdh1 and padh18a was obviously lower in embryos injected with zdhhc17 morpholino. Conclusion zdhhc17 is critical for zebrafish convergence and extension movements via regulating the expression of cadherin superfamily genes cdh1 and pcdh18a and affecting cell adhesion.

Key words: zdhhc17 , Palmitoylation , Convergence and extension movements , Cadherin superfamily , Whole mount in situ hybridization , Zebrafish

YANG Yang GAO Ming CHEN Xue-ran SHI Wei WANG Fen MA Bao-hua HAO Ai-jun* . Zdhhc17 regulating convergent and extension movements during zebrafish embryogenesis[J]. AAS, 2013, 44(2 ): 259-263.

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