Effects of Cdc20 mutation on growth of mouse embryonic fibroblast

doi:10.3969/j.issn.0529-1356.2014.03.016

Abstract

Abstract:

Objective Investigation of biological characteristics of Cdc20A ^AA/+APC^min/+mouse embryonic fibroblast(MEFs) indicate the effect of Cdc20AAA/+on growth of mouse embryonic fibroblast and the possible mechanism. Methods MEFs of Cdc20AAA/+APCmin/+, Cdc20 ^AAA/+ ,APCmin/+and WT genotype were harvested from embryos for analysis. The growth characteristics of Cdc20 ^AAA/+APC ^min/+ , Cdc20 ^AAA/+ ,APC ^min/+and WT mouse embryonic fibroblast were analyzed through growth curve analysis and foci formation assay. Separation of sister chromatid and the presence of aneuploid were detected by karyotype analysis. Results Cell proliferation assays showed that Cdc20 ^AAA/+APC ^min/+cells grew at an accelerated rate compared with APC ^min/+MEFs（P＜0.01）. Foci formation assay showed that the clone forming ability was significantly increased.Cdc20 ^AAA/+APC^min/+ MEFs showed a significant increase in the frequency of aneuploid compared with WT MEFs, which had a karyotype of 38 and contained prematurely separated sister chromatids. Conclusion Cdc20 carrying a null allele (Cdc20 ^AAA/+ ) may accelerate the growth and proliferation of APC ^min/+MEFs and present the growth characteristics of the tumor cells. The possible mechanism may be associated with chromosome instability.

Key words: Cdc20, APCmin/+, Embryonic fibroblast, Foci formation assay, Karyotype analysis, Chromosome, Instability, Mouse

FENG Ju-ling ZHAO Lei XIE Juan MO Ming-shu GUI Qing-jun YOU Yong ZHONG Hui WANG Li-sheng*. Effects of Cdc20 mutation on growth of mouse embryonic fibroblast[J]. AAS, 2014, 45(3): 383-387.

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