Effects of the uremic toxin p-cresylsulfate on human umbilical vein endotheilial cell function in vitro

doi:10.3969/j.issn.0529-1356.2014.02.013

Abstract

Abstract:

Objective To investigate the effect of a uremic toxin, p-cresylsulfate, on human umbilical vein endothelial cells (HUVECs) function. Methods HUVECs were incubated with various concentrations of p-cresylsulfate (10, 40, 80, and 160 mg/L) for up to 72 hours. Cell proliferation was determined using WST-1 assay. Tube formation assay was used to evaluate HUVECs function in vitro. Reactive oxygen species (ROS) production in HUVECs was measured by fluorescence microscopy. Inflammatory factor and adhesion molecule expressions were assessed by real time quantitative PCR(Real-time PCR) and Western blotting analysis. Results P-cresylsulfate had no anti-proliferative effects on HUVECs. Cells treated with concentrations of p-cresylsulfate in the range found in CKD patients had no observable impairment on tube formation. In contrast, p-cresylsulfate partially increased ROS production in HUVECs, while quantitative Real-time PCR and Western blotting revealed p-cresylsulfate treatment increase interleukin(IL)-1β, tumor necrosis factor(TNF-a), and intercelluar adhesion molecule\|1(ICAM-1) expressions in HUVECs. Conclusion Our study revealed that a uremic toxin, p-cresylsulfate, have pro-oxidant and pro-inflammatory effects on HUVECs, which indicates that p-cresylsulfate may be among the factors involved in the high incidence of atherosclerosis in patients with chronic kidney disease.

LIN Wei* XIA Jian-lan WANG Yi. Effects of the uremic toxin p-cresylsulfate on human umbilical vein endotheilial cell function in vitro[J]. AAS, 2014, (2): 216-220.

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