Acta Anatomica Sinica

AAS ›› 2014, Vol. ›› Issue (2): 239-241.doi: 10.3969/j.issn.0529-1356.2014.02.017

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Role of Notch signaling in platelet derived growth factor-BB induced proliferation of human pancreatic adenocarcinoma

MA Yong-chao FAN Wen-juan WU Hua WANG Fu-qing*   

  1. Department of Biochemistry and Molecular Biology, Basic Medical Institute, Luohe Medical College, He’nan  Luohe 46200, China
  • Received:2013-09-10 Revised:2013-11-17 Online:2014-04-06 Published:2014-04-06
  • Contact: WANG Fu-qing E-mail:wfq22000@yahoo.com.cn

Abstract:

Objective To clarify Notch signaling in platelet derived growth factor-BB(PDGF-BB) induced proliferation of human pancreatic adenocarcinoma (HPAC). Methods After being treated by PDGF-BB and (or) γ-secretase inhibitor DAPT, changes of HPAC cell proliferation were detected by MTT assay; Alterations of Notch level in HPAC cells were measured by Western blotting assay; Variations of capability in cardiolipin synthetic lecithin(CSL) binding to the promoter of target gene hes-1 were identified by chromatin immunoprecipitation (CHIP) assay. Results PDGF-BB facilitates the proliferation of HPAC cells, upregulates Notch level and enhances the capability in CSL binding to the promoter of hes-1. DAPT and PDGFR inhibitor treatment suppresses and invalidates PDGF-BB effection. Conclusion Notch-1 signaling plays an essential role in the modulation of PDGF-BB-induced proliferation of HPAC cells.