Acta Anatomica Sinica

AAS ›› 2014, Vol. 45 ›› Issue (1): 128-133.doi: 10.3969/j.issn.0529-1356.2014.01.025

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LIN28B gene polymorphisms in Ningxia Hui and Han ethnic people

PEI Li-guo1 QU Lei1 JIA Fei1 ZHANG Chuan1 GUO Meng-jing1 LU Hong1,2 HUO Zheng-hao1,2*   

  1. 1.Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance，Ministry of Education，Key Laboratory of Heredity and Reproduction of Ningxia, Yinchuan 750004, China;2.Department of Basic Medicine of Ningxia Medical University, Yinchuan 750004, China
  • Received:2013-07-08 Revised:2013-09-21 Online:2014-02-06 Published:2014-02-06
  • Contact: HUO Zheng-hao E-mail:medicalningxia@163.com

Abstract:

Objective To study the frequencies of rrs314277 and rs314276 allele and genotype distribution of the LIN28B gene polymorphisms in the Hui and Han people in Ningxia, and to analyze the distributions of LIN28B gene polymorphisms among different places and ethnicity. Methods A total of 705 subjects were from Ningxia Medical University, including 311 cases of boys, of which 144 cases of the Hui and 167 cases of the Han; 394 cases of girls, including the 188 cases of Hui and the 206 cases of Han were analyzed in this study. The two SNPs were examined by the sequencing methods. Results The genotypes frequencies and allele gene frequency of LIN28B gene in Ningxia Hui population were: male：rs314277 (AC:4.5%,CC:38.9%; A:2.3%, C:41.1%); rs314276 (AA:2.7%,AC:21.4%,CC:19.3%; A:13.4%,C:30.0%);female：rs314277(AA: 0.6%, AC:7.5%,CC:48.5%; A:4.4%, C:52.3%); rs314276(AA:3.3%,AC:28.9%, CC：24.4%; A:17.8%,C:38.9%). The genotypes frequencies and allele gene frequency of LIN28B gene in Ningxia Han population were: male：rs314277(AC:3.2%, CC:41.6%; A:1.6%, C:43.2%); rs314276(AA: 4.6%,AC:17.4%, CC:22.8%; A:13.3%, C:31.5%); female：rs314277(AC:4.6%,CC:50.7%; A:2.3%, C:52.9%); rs314276(AA: 4.8%, AC:18.8%, CC:31.6%; A:14.2%, C:41.0%). Conclusion This study showed a significant difference of allele between the Hui and Han ethnic of Ningxia population in rs314277(P<0.05), a significant difference of genotypes between the Hui and Han ethnic of Ningxia population in rs314276(P<0.05). No significant differentce is found between the male and female of Ningxia population in the two SNPs (P>0.05). The differences of distribution traits of genotype and allele frequencies between Ningxia population and other different etbnic population in LIN28B gene indicate that this two SNPs may have genetics characteristic in different ethnics.

Key words: LIN28B, Gene polymorphism, Sequencing, Hui ethnic, Han ethnic