Effect of stromal cell derived factor-1α and interleukin-1β on inducing vascular endothelial cells expressing lymphatic phenotype

doi:10.3969/j.issn.0529-1356.2014.03.017

Abstract

Abstract:

Objective To investigate the effect of stromal cell-derived factor-1α (SDF-1α) and interleukin(IL-1β) on inducing vascular endothelial cells to express lymphatic phenotype. Methods The CRL-1730 cell line was cultured and treated with SDF-1α or IL-1β. The expression of endothelial cell markers and lymphatic endothelial cell markers were investigated with Real-time PCR, Western blotting and immunocytochemistry. Results In CRL-1730 cell line, endothelial cell markers such as voln willebrand factor (vWF), VE-cadherin, vascular endothelial growth factor receptor(VEGFR)2, were dose dependently down-regulated after SDF-1α stimulation, while lymphatic phenotypes such as Prox-1, podoplanin and lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1), were dose-dependently up-regulated after SDF-1α stimulation. The changes of vWF, VEGFR2 and podoplanin, Prox-1, LYVE-1 expression after IL-1β stimulation was similar to that after SDF-1α while expression of VE-cadherin changed slightly. Conclusion SDF-1α and IL-1β are able to induce vascular endothelial cell expressing lymphatic phenotype.

Key words: Stromal cell-derived factor-1α, Interleukin-1β, Prospero ho-meobox protein-1, Vascular endothelial cell, Real-time PCR

SUO Ning WANG Xue-ying YANG Chun-lin ZHOU Hui LI Fei ZHANG Zong-pu WAN Fang-zhu TIAN Hua*. Effect of stromal cell derived factor-1α and interleukin-1β on inducing vascular endothelial cells expressing lymphatic phenotype[J]. AAS, 2014, 45(3): 388-392.

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